Home About Browse Search
Svenska


Is clinical effect of autologous conditioned serum in spontaneously occurring equine articular lameness related to ACS cytokine profile?

Marques-Smith, Patrick and Kallerud, Anne S. and Johansen, Grethe M. and Boysen, Preben and Jacobsen, Anna M. and Reitan, Karoline M. and Henriksen, Mia M. and Löfgren, Maria and Fjordbakk, Cathrine T. (2020). Is clinical effect of autologous conditioned serum in spontaneously occurring equine articular lameness related to ACS cytokine profile? BMC Veterinary Research. 16 , 181 , 1-9
[Journal article]

[img] PDF
718kB

Abstract

Background Biologic' therapies, such as autologous conditioned serum (ACS), are gaining popularity in treating orthopaedic conditions in equine veterinary medicine. Evidence is scarce regarding ACS constituents, and large inter-individual differences in cytokine and growth factor content have been demonstrated. The objective of the current study was to investigate the potential association between cytokine and growth factor content of ACS and clinical effect in harness racehorses with spontaneously occurring low-grade articular lameness. Horses received 3 intra-articular injections of ACS administered at approximately 2-week intervals. Lameness evaluation consisting of a trot-up with subsequent flexions tests was performed at inclusion and approximately 2 weeks after the last treatment (re-evaluation); horses were classified as responders when there was no detectable lameness on trot-up and a minimum of 50% reduction in flexion test scores at re-evaluation. Association between clinical outcome (responders vs. non-responders) and age, lameness grades at inclusion (both initial trot-up and after flexion tests), treatment interval, follow-up time and the ACS content of IL-1Ra, IGF-1 and TGF-beta was determined by regression modelling. Results Outcome analysis was available for 19 of 20 included horses; 11 responded to treatment whereas 8 did not. There was considerable inter-individual variability in cytokine/growth factor content of ACS, and in the majority of the horses, the level of IL-10, IL-1 beta and TNF-alpha was below the detection limit. In the final multivariate logistic regression model, ACS content of IGF-1 and IL-1Ra was significantly associated with clinical response (P = 0.01 and P = 0.03, respectively). No association with clinical response was found for the other tested variables. Conclusions The therapeutic benefit of ACS may be related to higher levels of IL-1Ra and IGF-1. Our study corroborates previous findings of considerable inter-individual variability of cytokine- and growth factor content in ACS.

Authors/Creators:Marques-Smith, Patrick and Kallerud, Anne S. and Johansen, Grethe M. and Boysen, Preben and Jacobsen, Anna M. and Reitan, Karoline M. and Henriksen, Mia M. and Löfgren, Maria and Fjordbakk, Cathrine T.
Title:Is clinical effect of autologous conditioned serum in spontaneously occurring equine articular lameness related to ACS cytokine profile?
Year of publishing :2020
Volume:16
Article number:181
Number of Pages:9
Publisher:BMC
ISSN:1746-6148
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Biologic therapies, Cytokines, Growth factors, Intra-articular treatment
URN:NBN:urn:nbn:se:slu:epsilon-p-106810
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106810
Additional ID:
Type of IDID
DOI10.1186/s12917-020-02391-7
Web of Science (WoS)000540851300001
ID Code:17299
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:20 Aug 2020 08:38
Metadata Last Modified:20 Aug 2020 08:38

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits