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Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate

Kianersi, Farzad and Abdollahi, Mohammad Reza and Mirzaie-asl, Asghar and Dastan, Dara and Rasheed, Faiza (2020). Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate. Scientific Reports. 10 , 8884 , 1-15
[Journal article]

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Abstract

Capparis spinosa is an edible medicinal plant which is considered as an excellent source of rutin. Rutin is a glycoside of the flavonoid quercetin that has been reported to have a beneficial role in controlling various diseases such as hypertension, arteriosclerosis, diabetes, and obesity. In this study, the partial cDNA of four genes involved in the rutin biosynthetic pathway including 4-coumaroyl CoA ligase (4CL), flavonoid 3'-hydroxylase (F3'H), flavonol synthase (FLS) and flavonol-3-O-glucoside L-rhamnosyltransferase (RT) were identified in C.spinosa plants for the first time. The protein sequences of these genes shared high similarity with the same proteins in other plant species. Subsequently, the expression patterns of these genes as well as rutin accumulation in C.spinosa leaves treated with different concentrations of salicylic acid (SA) and methyl jasmonate (MeJA) and also in different tissues of Caper plants treated with 100 mgL(-1) SA and 150 mu M MeJA were evaluated. The expression of all four genes was clearly up-regulated and rutin contents increased in response to MeJA and SA treatments after 24 h. The highest rutin contents (5.30 mgg(-1) DW and 13.27 mgg(-1) DW), as well as the highest expression levels of all four genes, were obtained using 100 mgL(-1) SA and 150 mu M MeJA, respectively. Among the different tissues, the highest rutin content was observed in young leaves treated with 150 mu M MeJA, which corresponded to the expression of related genes, especially RT, as a key gene in the rutin biosynthetic pathway. These results suggest that rutin content in various tissues of C. spinosa can be enhanced to a significant extent by MeJA and SA treatments and the gene expression patterns of rutin-biosynthesis-related genes are regulated by these elicitors.

Authors/Creators:Kianersi, Farzad and Abdollahi, Mohammad Reza and Mirzaie-asl, Asghar and Dastan, Dara and Rasheed, Faiza
Title:Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate
Year of publishing :2020
Volume:10
Article number:8884
Number of Pages:15
Publisher:Springer Nature
ISSN:2045-2322
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
URN:NBN:urn:nbn:se:slu:epsilon-p-106815
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106815
Additional ID:
Type of IDID
DOI10.1038/s41598-020-65815-2
Web of Science (WoS)000540482200076
ID Code:17300
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:20 Aug 2020 08:39
Metadata Last Modified:20 Aug 2020 08:39

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