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Fungal Diversity in the Phyllosphere of Pinus heldreichii H. Christ-An Endemic and High-Altitude Pine of the Mediterranean Region

Lazarevic, Jelena and Menkis, Audrius (2020). Fungal Diversity in the Phyllosphere of Pinus heldreichii H. Christ-An Endemic and High-Altitude Pine of the Mediterranean Region. Diversity. 12 , 172 , 1-16
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Abstract

Pinus heldreichii is a high-altitude coniferous tree species naturaly occurring in small and disjuncted populations in the Balkans and southern Italy. The aim of this study was to assess diversity and composition of fungal communities in living needles of P. heldreichii specifically focusing on fungal pathogens. Sampling was carried out at six different sites in Montenegro, where 2-4 year-old living needles of P. heldreichii were collected. Following DNA isolation, it was amplified using ITS2 rDNA as a marker and subjected to high-throughput sequencing. Sequencing resulted in 31,831 high quality reads, which after assembly were found to represent 375 fungal taxa. The detected fungi were 295 (78.7%) Ascomycota, 79 (21.0%) Basidiomycota and 1 (0.2%) Mortierellomycotina. The most common fungi were Lophodermium pinastri (12.5% of all high-quality sequences), L. conigenum (10.9%), Sydowia polyspora (8.8%), Cyclaneusma niveum (5.5%), Unidentified sp. 2814_1 (5.4%) and Phaeosphaeria punctiformis (4.4%). The community composition varied among different sites, but in this respect two sites at higher altitudes (harsh growing conditions) were separated from three sites at lower altitudes (milder growing conditions), suggesting that environmental conditions were among major determinants of fungal communities associated with needles of P. heldreichii. Trees on one study site were attacked by bark beetles, leading to discolouration and frequent dieback of needles, thereby strongly affecting the fungal community structure. Among all functional groups of fungi, pathogens appeared to be an important component of fungal communities in the phyllosphere of P. heldreichii, especially in those trees under strong abiotic and biotic stress.

Authors/Creators:Lazarevic, Jelena and Menkis, Audrius
Title:Fungal Diversity in the Phyllosphere of Pinus heldreichii H. Christ-An Endemic and High-Altitude Pine of the Mediterranean Region
Year of publishing :2020
Volume:12
Article number:172
Number of Pages:16
Publisher:MDPI
ISSN:1424-2818
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
Keywords:needle pathogens, high altitude forests, DNA metabarcoding, Montenegro
URN:NBN:urn:nbn:se:slu:epsilon-p-106823
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106823
Additional ID:
Type of IDID
DOI10.3390/d12050172
Web of Science (WoS)000541083200034
ID Code:17304
Faculty:S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:20 Aug 2020 08:45
Metadata Last Modified:20 Aug 2020 08:45

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