Home About Browse Search
Svenska


Sodium Ascorbate as a Quorum-Sensing Inhibitor Leads to Decreased Virulence in Vibrio campbellii

Han, Biao and Zheng, Xiaoting and Baruah, Kartik and Bossier, Peter (2020). Sodium Ascorbate as a Quorum-Sensing Inhibitor Leads to Decreased Virulence in Vibrio campbellii. Frontiers in Microbiology. 11 , 1054 , 1-10
[Journal article]

[img] PDF
735kB

Abstract

Vibrio campbelliiis one of the major bacterial pathogens for animals reared in aquaculture, affecting both vertebrates and invertebrates, and causes significant economic losses. It is now evident that the expressions of virulence factors in this pathogen are regulated by the density of the bacterial population. This type of regulation, termed quorum sensing (QS), is mediated by extracellular signal molecules called autoinducers. In this study, the impact of sodium ascorbate (NaAs) on the virulence ofV. campbelliiwas investigated under bothin vitroandin vivoconditions, to develop a natural anti-infective strategy to containV. campbelliiinfection in aquacultured animals. Results showed that NaAs significantly decreased swimming motility, biofilm production, and the production of virulence enzymes, such as lipase, caseinase, phospholipase, and hemolysin inV. campbellii. Consistent with this, pretreatment ofV. campbelliiwith NaAs before inoculation into the rearing water resulted in significantly increased survival of gnotobiotic brine shrimp larvae, when compared to larvae challenged with untreatedV. campbellii. Furthermore, NaAs could interfere with QS-regulated bioluminescence inV. campbellii, suggesting the QS-inhibitory activity largely determines the protective effect of NaAs toward the brine shrimp. In essence, due to the potent anti-virulence effects observed inin vitrostudies and the clinical brine shrimp-V. campbelliiinfection model, NaAs constitute a promising novel strategy for the control ofV. campbelliiinfections in aquaculture.

Authors/Creators:Han, Biao and Zheng, Xiaoting and Baruah, Kartik and Bossier, Peter
Title:Sodium Ascorbate as a Quorum-Sensing Inhibitor Leads to Decreased Virulence in Vibrio campbellii
Year of publishing :2020
Volume:11
Article number:1054
Number of Pages:10
Publisher:Frontiers
ISSN:1664-302X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:sodium ascorbate, quorum sensing inhibitor, virulence, Vibrio campbellii, Artemia
URN:NBN:urn:nbn:se:slu:epsilon-p-106890
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106890
Additional ID:
Type of IDID
DOI10.3389/fmicb.2020.01054
Web of Science (WoS)000543333100001
ID Code:17323
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:25 Aug 2020 11:44
Metadata Last Modified:25 Aug 2020 11:44

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits