Home About Browse Search
Svenska


Effects of cashew gum and nanoparticles on cooled stallion semen

Loureiro, Kahynna Cavalcante and Lima Verde, Isabel and Johannisson, Anders and Ntallaris, Theodoros and Jager, Alessandro and Stepanek, Petr and da Costa Mendonca, Marcelo and Severino, Patricia and Morrell, Jane (2020). Effects of cashew gum and nanoparticles on cooled stallion semen. Acta Veterinaria Scandinavica. 62 , 31 , 1-9
[Journal article]

[img] PDF
1MB

Abstract

Background: Cryopreservation of stallion spermatozoa tends to cause plasma membrane damage due to the low ratio of cholesterol to phospholipids. Gums have been suggested as an alternative cryoprotectant to glycerol for stallion spermatozoa. Therefore, the present experiment was designed to verify whether the effect of addition of cashew gum (CG), or nanoparticles (NP) containing CG, to the extender before cooling on sperm quality in stallion semen. Ejaculates from 6 stallions were extended and split between six treatment groups (control, a-tocopherol [TOC], CG1, CG0.5, NP1 and NP0.5), stored in cryotubes at 4 degrees C.
Results: Aliquots were analysed by computer-assisted sperm motility analysis on the day of collection, and after 24 h and 48 h of cold storage. After 48 h, the total motility with NP1 (78.53 + 6.31%) was similar to control 85.79 + 6.31% at 0 h. The same pattern was observed for progressive motility. Membrane integrity assessed by flow cytometer was similar between control, TOC and G1 at all storage times. The DNA fragmentation in the control group increased at all time points, whereas chromatin integrity was maintained after 24 h in TOC and NP0.5 compared to 0 h. There was no increase in the proportion of live spermatozoa producing hydrogen peroxide, but there was a tendency for an increased proportion of spermatozoa in the live superoxide category in CG1 after 24 h cooled storage.
Conclusions: The addition of CG or CG-derived NP to extender for stallion semen was not harmful to the sperm cells.

Authors/Creators:Loureiro, Kahynna Cavalcante and Lima Verde, Isabel and Johannisson, Anders and Ntallaris, Theodoros and Jager, Alessandro and Stepanek, Petr and da Costa Mendonca, Marcelo and Severino, Patricia and Morrell, Jane
Title:Effects of cashew gum and nanoparticles on cooled stallion semen
Year of publishing :2020
Volume:62
Article number:31
Number of Pages:9
Publisher:BMC
ISSN:0044-605X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Cashew gum, Cooling, Nanoparticles, Semen, Stallion
URN:NBN:urn:nbn:se:slu:epsilon-p-106925
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106925
Additional ID:
Type of IDID
DOI10.1186/s13028-020-00530-6
Web of Science (WoS)000544920900001
ID Code:17334
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:28 Aug 2020 07:40
Metadata Last Modified:28 Aug 2020 07:40

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits