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Bovine acute phase proteins in milk

haptoglobin and serum amyloid A as potential biomarkers for milk quality

Åkerstedt, Maria (2008). Bovine acute phase proteins in milk. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2008:16
ISBN 978-91-85913-49-7
[Doctoral thesis]

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Abstract

The composition and quality of the raw milk is essential for the dairy industry and since only healthy cows produce milk of high quality, it is important with prospering cows. The most important and common disease among dairy cows is mastitis (inflammation of the udder). Mastitis is not only an animal welfare problem, but also results in impaired milk quality, reduced product yield, higher production costs and consequently a higher price for the consumer. The most common way to detect subclinical mastitis is by measuring the somatic cell count (SCC). The SCC is also an important parameter in milk payment systems, highly affecting the price to the producer. Since SCC is influenced by other factors than mastitis, e.g. lactation number, stage of lactation, stress etc., there is a need for new biomarkers for detection of subclinical mastitis as well as for raw milk quality. The aim of this thesis was to obtain further knowledge about the occurrence of the two major acute phase proteins (APP) in bovine milk, haptoglobin (Hp) and serum amyloid A (SAA), and to evaluate their potential as biomarkers for raw milk quality. For the first time, a method for analysis of Hp using an optical biosensor based on surface plasmon resonance (SPR) technology was developed. The method is fully automated with no need for sample preparation before analysis and each sample requires approximately 8 minutes. The occurrence of Hp and SAA in quarter, cow composite and bulk tank milk samples were investigated and the results showed that APP could be detected in all types of samples. In general, detectable levels of APP in milk were related to high SCC, probably originating from cows with subclinical mastitis. In general, samples containing APP had lower casein content, casein number (casein in relation to total protein) and lactose but also increased whey protein content or increased proteolysis. Hp and SAA were suggested to be useful biomarkers for milk quality, especially the protein quality. To our knowledge, this thesis is the first describing Hp and SAA as potential biomarkers for raw milk quality.

Authors/Creators:Åkerstedt, Maria
Title:Bovine acute phase proteins in milk
Subtitle:haptoglobin and serum amyloid A as potential biomarkers for milk quality
Year of publishing :2008
Volume:2008:16
Number of Pages:55
Papers/manuscripts:
NumberReferences
ALLI. Åkerstedt, M., Björck, L., Persson Waller, K. & Sternesjö, Å. 2006. Biosensor assay for determination of haptoglobin in bovine milk. Journal of Dairy Research 73, 299-305. II. Åkerstedt, M., Persson Waller, K. & Sternesjö, Å. 2007. Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples. Journal of Dairy Research 74, 198-203. III. Åkerstedt, M., Persson Waller, K., Bach Larsen L., Forsbäck, L. & Sternesjö, Å. 2008. Relationship between haptoglobin and serum amyloid A in milk and milk quality. Accepted for publication in International Dairy Journal. doi:10.1016/j.idairyj.2008.01.002 IV. Åkerstedt, M., Persson Waller, K. & Sternesjö, Å. 2008. Haptoglobin and serum amyloid A in bulk tank milk in relation to raw milk quality. Submitted.
Place of Publication:Uppsala
ISBN for printed version:978-91-85913-49-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:dairy cows, cow milk, quality, proximate composition, proteins, casein, somatic cell count, mastitis, biosensors, proteolysis
Keywords:bovine, dairy cow, milk quality, acute phase proteins, haptoglobin, serum amyloid A, somatic cell count, mastitis, protein composition, casein content, proteolysis
URN:NBN:urn:nbn:se:slu:epsilon-2170
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2170
ID Code:1735
Department:(NL, NJ) > Dept. of Food Science (until 161231)
Deposited By: Maria Åkerstedt
Deposited On:11 Apr 2008 00:00
Metadata Last Modified:02 Dec 2014 10:13

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