Bergman, Daniel and Bäckström, Camilla and Hansson-Hamlin, Helene and Larsson, Anders and Ström Holst, Bodil
(2020).
Pre-existing canine anti-IgG antibodies: implications for immunotherapy, immunogenicity testing and immunoassay analysis.
Scientific Reports. 10
, 12696
, 1-10
[Research article]
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Abstract
One of the most enigmatic features of humoral immunity is the prevalent presence of circulating autoantibodies against IgG. These autoantibodies consist of several subsets, including rheumatoid factors, anti-Fab/anti-F(ab′)2-autoantibodies, and anti-idiotypic antibodies. Anti-IgG autoantibodies can impair the safety and efficacy of therapeutic antibodies and interfere with immunogenicity tests in clinical trials. They can also cross-react with allospecific IgG, presenting as heterophilic antibodies that interfere with diagnostic immunoassays. Owing to these factors, recent years have seen a resurgent interest in anti-IgG autoantibodies, but their underlying clinical significance, as well as biological roles and origins, remain opaque. Increased knowledge about canine anti-IgG autoantibodies could facilitate the development of canine immunotherapies and help in understanding and counteracting immunoassay interference. This study investigated the clinical significance and interconnection of heterophilic antibodies, anti-Fab, and anti-F(ab′)2-autoantibodies in dogs. We performed a 2-year prospective follow-up of dogs with heterophilic antibodies and analyzed serum for anti-Fab and anti-F(ab′)2-autoantibodies. Canine heterophilic antibodies can persist for at least 2 years in serum. A widespread occurrence of anti-Fab and anti-F(ab′)2-autoantibodies was found, with reactivity to cryptic epitopes in the IgG hinge region and sporadic cross-reactivity with mouse IgG. Canine anti-Fab and anti-F(ab′)2-autoantibodies are thus potential sources of clinical immunogenicity and immunoassay interference.
Authors/Creators: | Bergman, Daniel and Bäckström, Camilla and Hansson-Hamlin, Helene and Larsson, Anders and Ström Holst, Bodil | ||||
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Title: | Pre-existing canine anti-IgG antibodies: implications for immunotherapy, immunogenicity testing and immunoassay analysis | ||||
Series Name/Journal: | Scientific Reports | ||||
Year of publishing : | 2020 | ||||
Volume: | 10 | ||||
Article number: | 12696 | ||||
Number of Pages: | 10 | ||||
Publisher: | Springer Nature | ||||
ISSN: | 2045-2322 | ||||
Language: | English | ||||
Publication Type: | Research article | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Immunology in the medical area (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Clinical Laboratory Medicine | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-106956 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106956 | ||||
Additional ID: |
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ID Code: | 17381 | ||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||
Department: | (VH) > Dept. of Clinical Sciences | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 02 Sep 2020 09:43 | ||||
Metadata Last Modified: | 15 Jan 2021 19:18 |
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