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Chemosensation in Aedes aegypti: from molecular response profiles to host-seeking behaviour

Tallon, Anaïs (2020). Chemosensation in Aedes aegypti: from molecular response profiles to host-seeking behaviour. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2020:44
ISBN 978-91-7760-606-2
eISBN 978-91-7760-607-9
[Doctoral thesis]

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Abstract

The female mosquito Aedes aegypti is the primary urban vector of various highly infectious neglected tropical diseases. Disease transmission relies heavily on odourmediated behaviours, including e.g., sugar- and host-seeking, which are crucial during the mosquito life cycle and has dire impacts on vectorial capacity. Olfaction is, one of the most sophisticated sensory systems, and is the sense by which insects locate and discriminate biologically-relevant odorant volatiles. In female mosquitoes, host-seeking is generally not observed before 2-3 days following adult emergence. Of note, males have also been shown to respond to and orient towards host-associated cues. Vector-borne pathogens can alter the behaviour and physiology of their host, consequently increasing the risk of disease transmission. Thus, the modulation of host-seeking depends on multiple factors, including e.g., age, sex, and infection by a pathogen, which is the main focus of this thesis. During my PhD, I have shown that the modulation of the onset of host-seeking coincides with changes in transcript abundance of chemosensory-related genes expressed in the antennae of newly-emerged mosquitoes. Of these genes, one particular odorant receptor, OR117, exhibits a decrease in transcript abundance as female mosquitoes become capable of host-seeking. Later, I have functionally characterised OR117, together with another odorant receptor-of-interest, OR103, which has previously been shown to be more abundant in the human-preferring subspecies of Ae. aegypti. Both OR117 and OR03 are tuned to monoterpenes, and their potential role in the transition between sugar- to host-seeking and human avoidance, respectively, is discussed. Furthermore, I have demonstrated that the locomotion and host-seeking is modulated in dengue-infected females in a time-dependent manner, i.e. depending on the time post-infection, and coincides with changes in transcript abundance of genes involved in neural signalling pathways. A better understanding of the modulation of odour-mediated behaviours in mosquitoes is crucial for the development of efficient control strategies against disease vector control.

Authors/Creators:Tallon, Anaïs
Title:Chemosensation in Aedes aegypti: from molecular response profiles to host-seeking behaviour
Year of publishing :2020
Number:2020:44
Number of Pages:163
Publisher:Department of Plant Protection Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-606-2
ISBN for electronic version:978-91-7760-607-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Behavioural Sciences Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Other Biological Topics
Keywords:chemical ecology, chemosensory-related genes, dengue, deorphanization, electrophysiology, heterologous expression, mosquitoes, olfaction
URN:NBN:urn:nbn:se:slu:epsilon-p-107039
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107039
ID Code:17392
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:19 Aug 2020 06:44
Metadata Last Modified:19 Aug 2020 06:44

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