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Production of moth sex pheromone precursors in Nicotiana spp.: a worthwhile new approach to pest control

Xia, Yi-Han and Ding, Bao-Jian and Wang, Hong-Lei and Hofvander, Per and Jarl-Sunesson, Carin and Lofstedt, Christer (2020). Production of moth sex pheromone precursors in Nicotiana spp.: a worthwhile new approach to pest control. Journal of Pest Science. 93 , 1333-1346
[Journal article]

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Abstract

Pheromones are environmentally friendly alternatives to traditional pesticides for pest control. They are widely applied for insect monitoring, mating disruption and mass trapping.Nicotiana benthamianaandN. tabacumare potential green biomass production platforms of moth sex pheromones. Using these twoNicotianaspecies as plant factories, we expressed biosynthetic genes of plant and insect origin in leaf tissue. Moth sex pheromone precursors (E)-11-tetradecenoic acid, (Z)-11-tetradecenoic acid and (Z)-11-hexadecenoic acid were produced by introducing the acyl-ACP thioesterasesCpuFatB1fromCuphea pulcherrimaorCpaFatB2fromC. palustrisand the fatty acyl desaturasesAve increment 11fromArgyrotaenia velutinana,CpaE11fromChoristoneura parallelaorAtr increment 11fromAmyelois transitella, under the control of CaMV-35S promoter. Among theNicotianaspp. transformants, the best line produced (Z)-11-hexadecenoic acid at 17.6% of total fatty acids in leaves, during flowering stage, corresponding to 335 mu g of (Z)-11-hexadecenoic acid per gram of fresh leaf. The (Z)-11-hexadecenoic acid production lines fromN. benthamianawere selected for further propagation to obtain homozygous lines. In theN. benthamianaT2 generation, the production quantity of (Z)-11-hexadecenoic acid was stable. Our study demonstrates the feasibility of stable transformation ofN. benthamianafor production of moth pheromone precursors in vegetative tissue.

Authors/Creators:Xia, Yi-Han and Ding, Bao-Jian and Wang, Hong-Lei and Hofvander, Per and Jarl-Sunesson, Carin and Lofstedt, Christer
Title:Production of moth sex pheromone precursors in Nicotiana spp.: a worthwhile new approach to pest control
Year of publishing :2020
Volume:93
Page range:1333-1346
Number of Pages:14
Publisher:Springer
ISSN:1612-4758
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Pest control, Insect pheromone precursors, Nicotianaspp, Acyl-ACP thioesterase, Fatty acyl desaturase, Stable transformation
URN:NBN:urn:nbn:se:slu:epsilon-p-106882
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106882
Additional ID:
Type of IDID
DOI10.1007/s10340-020-01250-6
Web of Science (WoS)000541315900002
ID Code:17415
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:07 Sep 2020 09:42
Metadata Last Modified:07 Sep 2020 09:42

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