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Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways

Rubnawaz, Samina and Kayani, Waqas Khan and Mahmood, Rashid and Mirza, Bushra (2020). Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways. Turkish Journal Of Botany. 44 , 410-426
[Journal article]

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Abstract

The rol oncogenes of Agrobacterium rhizogenes enhance the production of medicinally important compounds in plants and provide a first barrier against the overproduction of reactive oxygen species during biotic and abiotic stress. 'this study was designed to evaluate the expression of genes involved in biosynthetic pathways and their impact on metabolic contents and environmental stress tolerance in regenerated Ajuga bracteosa Wall. ex Benth. After successful transformation, real-time quantitative PCR confirmed the increased expression (1.94-6.59-fold) of HMGR, HDS, FDS, PAL, and TTG1 genes in transgenic lines. Furthermore, GC-MS coupled with principal component analysis revealed diverse concentrations of 97 metabolites in A. bracteosa. Transgenic lines showed greater survival under multiple stresses. This was revealed by significant chlorophyll content (8.13-21 mu moles/m(2)), higher quantum efficiency of PSII (F-v/F-m), and the performance index (PIabs) value. Similarly, catalase and peroxidase enzyme activities were enhanced during extreme drought (300-400 mM mannitol) and salinity (150-200 mM NaCl) conditions, compared to untransformed control. Wild type control plant leaves were completely necrotized by Aspergillus fumigatus (FCBP 66) and Fusarium solani (FCBP 0291), whereas transformed leaves had improved antifungal resistance. In conclusion, our data suggest that rolABC genes have a significant impact on the synthesis of metabolites involved in enhancing multistress tolerance in A. bracteosa.

Authors/Creators:Rubnawaz, Samina and Kayani, Waqas Khan and Mahmood, Rashid and Mirza, Bushra
Title:Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways
Year of publishing :2020
Volume:44
Page range:410-426
Number of Pages:19
Publisher:TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
ISSN:1300-008X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:Ajuga bracteosa, rolABC, real-time quantitative PCR, GC-MS, principal component analysis, biotic and abiotic stress
URN:NBN:urn:nbn:se:slu:epsilon-p-107153
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107153
Additional ID:
Type of IDID
DOI10.3906/bot-2002-13
Web of Science (WoS)000550037100005
ID Code:17436
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:14 Sep 2020 08:49
Metadata Last Modified:14 Sep 2020 08:49

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