Rubnawaz, Samina and Kayani, Waqas Khan and Mahmood, Rashid and Mirza, Bushra
(2020).
Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways.
Turkish Journal Of Botany. 44
, 410-426
[Journal article]
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Abstract
The rol oncogenes of Agrobacterium rhizogenes enhance the production of medicinally important compounds in plants and provide a first barrier against the overproduction of reactive oxygen species during biotic and abiotic stress. 'this study was designed to evaluate the expression of genes involved in biosynthetic pathways and their impact on metabolic contents and environmental stress tolerance in regenerated Ajuga bracteosa Wall. ex Benth. After successful transformation, real-time quantitative PCR confirmed the increased expression (1.94-6.59-fold) of HMGR, HDS, FDS, PAL, and TTG1 genes in transgenic lines. Furthermore, GC-MS coupled with principal component analysis revealed diverse concentrations of 97 metabolites in A. bracteosa. Transgenic lines showed greater survival under multiple stresses. This was revealed by significant chlorophyll content (8.13-21 mu moles/m(2)), higher quantum efficiency of PSII (F-v/F-m), and the performance index (PIabs) value. Similarly, catalase and peroxidase enzyme activities were enhanced during extreme drought (300-400 mM mannitol) and salinity (150-200 mM NaCl) conditions, compared to untransformed control. Wild type control plant leaves were completely necrotized by Aspergillus fumigatus (FCBP 66) and Fusarium solani (FCBP 0291), whereas transformed leaves had improved antifungal resistance. In conclusion, our data suggest that rolABC genes have a significant impact on the synthesis of metabolites involved in enhancing multistress tolerance in A. bracteosa.
Authors/Creators: | Rubnawaz, Samina and Kayani, Waqas Khan and Mahmood, Rashid and Mirza, Bushra | ||||||
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Title: | Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways | ||||||
Year of publishing : | 2020 | ||||||
Volume: | 44 | ||||||
Page range: | 410-426 | ||||||
Number of Pages: | 19 | ||||||
Publisher: | TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY | ||||||
ISSN: | 1300-008X | ||||||
Language: | English | ||||||
Publication Type: | Journal article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany | ||||||
Keywords: | Ajuga bracteosa, rolABC, real-time quantitative PCR, GC-MS, principal component analysis, biotic and abiotic stress | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-107153 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107153 | ||||||
Additional ID: |
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ID Code: | 17436 | ||||||
Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||||
Department: | (LTJ, LTV) > Department of Plant Breeding (from 130101) | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 14 Sep 2020 08:49 | ||||||
Metadata Last Modified: | 15 Jan 2021 19:45 |
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