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Diazotroph Genomes and Their Seasonal Dynamics in a Stratified Humic Bog Lake

Fernandez, Leyden and Peura, Sari and Eiler, Alexander and Linz, Alexandra M. and McMahon, Katherine D. and Bertilsson, Stefan (2020). Diazotroph Genomes and Their Seasonal Dynamics in a Stratified Humic Bog Lake. Frontiers in Microbiology. 11 , 1500 , 1-16
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Abstract

Aquatic N-fixation is generally associated with the growth and mass development ofCyanobacteriain nitrogen-deprived photic zones. However, sequenced genomes and environmental surveys suggest active aquatic N-fixation also by many non-cyanobacterial groups. Here, we revealed the seasonal variation and genomic diversity of potential N-fixers in a humic bog lake using metagenomic data andnifgene clusters analysis. Groups with diazotrophic operons were functionally divergent and includedCholorobi,Geobacter,Desulfobacterales,Methylococcales, andAcidobacteria. In addition tonifH(a gene that encodes the dinitrogenase reductase component of the molybdenum nitrogenase), we also identified sequences corresponding to vanadium and iron-only nitrogenase genes. Within theChlorobipopulation, the nitrogenase (nifH) cluster was included in a well-structured retrotransposon. Furthermore, the presence of light-harvesting photosynthesis genes implies that anoxygenic photosynthesis may fuel nitrogen fixation under the prevailing low-irradiance conditions. The presence ofrnfgenes (related to the expression of H+/Na+-translocating ferredoxin: NAD+ oxidoreductase) inMethylococcalesandDesulfobacteralessuggests that other energy-generating processes may drive the costly N-fixation in the absence of photosynthesis. The highly reducing environment of the anoxic bottom layer of Trout Bog Lake may thus also provide a suitable niche for active N-fixers and primary producers. While future studies on the activity of these potential N-fixers are needed to clarify their role in freshwater nitrogen cycling, the metagenomic data presented here enabled an initial characterization of previously overlooked diazotrophs in freshwater biomes.

Authors/Creators:Fernandez, Leyden and Peura, Sari and Eiler, Alexander and Linz, Alexandra M. and McMahon, Katherine D. and Bertilsson, Stefan
Title:Diazotroph Genomes and Their Seasonal Dynamics in a Stratified Humic Bog Lake
Year of publishing :2020
Volume:11
Article number:1500
Number of Pages:16
Publisher:FRONTIERS MEDIA SA
ISSN:1664-302X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:diazotrophs, N-fixation, lake, hypolimnion, nifHgene
URN:NBN:urn:nbn:se:slu:epsilon-p-107200
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107200
Additional ID:
Type of IDID
DOI10.3389/fmicb.2020.01500
Web of Science (WoS)000553270700001
ID Code:17449
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:14 Sep 2020 11:12
Metadata Last Modified:14 Sep 2020 11:12

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