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Properties of Biomimetic Artificial Spider Silk Fibers Tuned by PostSpin Bath Incubation

Greco, Gabriele and Francis, Juanita and Arndt, Tina and Schmuck, Benjamin and Backlund, Fredrik G. and Barth, Andreas and Johansson, Jan and Pugno, Nicola M. and Rising, Anna (2020). Properties of Biomimetic Artificial Spider Silk Fibers Tuned by PostSpin Bath Incubation. Molecules (Basel, Switzerland). 25 , 3248 , 1-11
[Journal article]

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Abstract

Efficient production of artificial spider silk fibers with properties that match its natural counterpart has still not been achieved. Recently, a biomimetic process for spinning recombinant spider silk proteins (spidroins) was presented, in which important molecular mechanisms involved in native spider silk spinning were recapitulated. However, drawbacks of these fibers included inferior mechanical properties and problems with low resistance to aqueous environments. In this work, we show that >= 5 h incubation of the fibers, in a collection bath of 500 mM NaAc and 200 mM NaCl, at pH 5 results in fibers that do not dissolve in water or phosphate buffered saline, which implies that the fibers can be used for applications that involve wet/humid conditions. Furthermore, incubation in the collection bath improved the strain at break and was associated with increased beta-sheet content, but did not affect the fiber morphology. In summary, we present a simple way to improve artificial spider silk fiber strain at break and resistance to aqueous solvents.

Authors/Creators:Greco, Gabriele and Francis, Juanita and Arndt, Tina and Schmuck, Benjamin and Backlund, Fredrik G. and Barth, Andreas and Johansson, Jan and Pugno, Nicola M. and Rising, Anna
Title:Properties of Biomimetic Artificial Spider Silk Fibers Tuned by PostSpin Bath Incubation
Year of publishing :2020
Volume:25
Article number:3248
Number of Pages:11
Publisher:MDPI
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Polymer Chemistry
Keywords:fiber, tensile testing, mechanical properties, spinning
URN:NBN:urn:nbn:se:slu:epsilon-p-107201
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107201
Additional ID:
Type of IDID
DOI10.3390/molecules25143248
Web of Science (WoS)000554165100001
ID Code:17451
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:14 Sep 2020 11:13
Metadata Last Modified:14 Sep 2020 11:13

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