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Bull seminal plasma stimulates in vitro production of TGF-β, IL-6 and IL-8 from bovine endometrial epithelial cells, depending on dose and bull fertility

Morrell, Jane and Nongbua, Thanapol and Guo, Yongzhi and Ntallaris, Theodoros and Rubér, Marie and Rodriguez-Martinez, Heriberto and Humblot, Patrice and Morrell, Jane (2020). Bull seminal plasma stimulates in vitro production of TGF-β, IL-6 and IL-8 from bovine endometrial epithelial cells, depending on dose and bull fertility. Journal of Reproductive Immunology. 142 , 103179
[Journal article]

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Abstract

Seminal plasma (SP) regulates immune responses in the female reproductive tract through specific cytokines. Itis not known whether SP from high fertility bulls (H) differs from SP from low fertility bulls (L). In this study, thecytokine response of bovine endometrial epithelial cells (bEEC) in culture was investigated after challenge withSP from two bulls of below average (L) or three bulls of above average fertility (H). The bEECs were challengedwith 1% or 4% SP fromL- or H-fertility bulls (L1, L4, H1, H4, respectively) or 1% or 4% PBS as control (C1, C4)for 72 h. The culture media were analysed for concentrations (pg/million cells) of transforming growth factorbeta (TGF-β1, TGF-β2 and TGF-β3) by Luminex, and Interleukin 6 and 8 (IL-6, IL-8) by ELISA. Challenge sig-nificantly affected production of TGF-ß1, TGF-ß2 and IL-8 compared to controls and was affected by bull fertility(p < 0.0001), SP concentration (p < 0.0001) and their interaction (p < 0.0001). A higher production of TGF-β1, TGF-β2 and IL-8 (p < 0.0001), and also IL-6 (p < 0.01), resulted from challenge with high doses of SP,being higher for L than H (p < 0.05). For TGF-β3, fertility of bull (p < 0.05). For TGF-B3, fertility of bull(p < 0.05) and the interaction between fertility and concentration of SP were significant (p < 0.01). In con-clusion, 4% SP from L bulls stimulated more TGF-β1, TGF-β2, TGF-β3, IL-6 and IL-8 production than SP from Hbulls, indicating that stimulation of the endometrium is relevant for fertility. Seminal plasma from high fertilitybulls seems to affect cytokine productionin uteropositively in inseminated cows.

Authors/Creators:Morrell, Jane and Nongbua, Thanapol and Guo, Yongzhi and Ntallaris, Theodoros and Rubér, Marie and Rodriguez-Martinez, Heriberto and Humblot, Patrice and Morrell, Jane
Title:Bull seminal plasma stimulates in vitro production of TGF-β, IL-6 and IL-8 from bovine endometrial epithelial cells, depending on dose and bull fertility
Year of publishing :2020
Volume:142
Article number:103179
Number of Pages:7
Publisher:Elsevier
ISSN:0165-0378
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:Uterus, Seminal plasma proteins, Artificial insemination, High fertility bulls, Low fertility bulls, Endometrium
URN:NBN:urn:nbn:se:slu:epsilon-p-107168
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107168
Additional ID:
Type of IDID
DOI10.1016/j.jri.2020.103179
ID Code:17487
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:22 Sep 2020 08:42
Metadata Last Modified:22 Sep 2020 08:42

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