Morrell, Jane and Nongbua, Thanapol and Guo, Yongzhi and Ntallaris, Theodoros and Rubér, Marie and Rodriguez-Martinez, Heriberto and Humblot, Patrice and Morrell, Jane
(2020).
Bull seminal plasma stimulates in vitro production of TGF-β, IL-6 and IL-8 from bovine endometrial epithelial cells, depending on dose and bull fertility.
Journal of Reproductive Immunology. 142
, 103179
[Journal article]
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Abstract
Seminal plasma (SP) regulates immune responses in the female reproductive tract through specific cytokines. Itis not known whether SP from high fertility bulls (H) differs from SP from low fertility bulls (L). In this study, thecytokine response of bovine endometrial epithelial cells (bEEC) in culture was investigated after challenge withSP from two bulls of below average (L) or three bulls of above average fertility (H). The bEECs were challengedwith 1% or 4% SP fromL- or H-fertility bulls (L1, L4, H1, H4, respectively) or 1% or 4% PBS as control (C1, C4)for 72 h. The culture media were analysed for concentrations (pg/million cells) of transforming growth factorbeta (TGF-β1, TGF-β2 and TGF-β3) by Luminex, and Interleukin 6 and 8 (IL-6, IL-8) by ELISA. Challenge sig-nificantly affected production of TGF-ß1, TGF-ß2 and IL-8 compared to controls and was affected by bull fertility(p < 0.0001), SP concentration (p < 0.0001) and their interaction (p < 0.0001). A higher production of TGF-β1, TGF-β2 and IL-8 (p < 0.0001), and also IL-6 (p < 0.01), resulted from challenge with high doses of SP,being higher for L than H (p < 0.05). For TGF-β3, fertility of bull (p < 0.05). For TGF-B3, fertility of bull(p < 0.05) and the interaction between fertility and concentration of SP were significant (p < 0.01). In con-clusion, 4% SP from L bulls stimulated more TGF-β1, TGF-β2, TGF-β3, IL-6 and IL-8 production than SP from Hbulls, indicating that stimulation of the endometrium is relevant for fertility. Seminal plasma from high fertilitybulls seems to affect cytokine productionin uteropositively in inseminated cows.
Authors/Creators: | Morrell, Jane and Nongbua, Thanapol and Guo, Yongzhi and Ntallaris, Theodoros and Rubér, Marie and Rodriguez-Martinez, Heriberto and Humblot, Patrice and Morrell, Jane | ||||
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Title: | Bull seminal plasma stimulates in vitro production of TGF-β, IL-6 and IL-8 from bovine endometrial epithelial cells, depending on dose and bull fertility | ||||
Year of publishing : | 2020 | ||||
Volume: | 142 | ||||
Article number: | 103179 | ||||
Number of Pages: | 7 | ||||
Publisher: | Elsevier | ||||
ISSN: | 0165-0378 | ||||
Language: | English | ||||
Publication Type: | Journal article | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science. | ||||
Keywords: | Uterus, Seminal plasma proteins, Artificial insemination, High fertility bulls, Low fertility bulls, Endometrium | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-107168 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107168 | ||||
Additional ID: |
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ID Code: | 17487 | ||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||
Department: | (VH) > Dept. of Clinical Sciences | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 22 Sep 2020 08:42 | ||||
Metadata Last Modified: | 15 Jan 2021 19:44 |
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