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Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

Stenberg, Hedvig and Jacobson, Magdalena and Malmberg, Maja (2020). Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II. BMC Veterinary Research. 16 , 260
[Journal article]

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Abstract

Background Congenital tremor (CT) type A-II is a neurological disorder characterized by tremor of the head and body of newborn piglets. The suggested causative agent of the disease is the recently found atypical porcine pestivirus (APPV). The virus has been detected in piglets suffering from congenital tremor in central Europe, South and North America and in China but no studies has so far been performed in the Nordic countries. The overarching goal of this study was to investigate if APPV is present in the brain tissue of Swedish piglets suffering from congenital tremor. From June 2017 - June 2018, 15 piglets from four Swedish farms with ongoing outbreaks of congenital tremor and 13 piglets with splay leg originating from four different farms, were investigated for presence of APPV RNA in brain tissue. Matched healthy control piglets (n=8) were also investigated. Two APPV-specific RT-qPCR methods targeting the NS3 and NS5B region, respectively, were used. A retrospective study was performed on material from Swedish piglets with congenital tremor sampled in 2004 (n=11) and 2011/2012 (n=3) using the described APPV-specific RT-qPCR methods. The total number of piglets with signs of CT in this study was 29.
Results Atypical porcine pestivirus-RNA was detected in 93% (27/29) of the piglets suffering from congenital tremor. All piglets with congenital tremor from 2004 (n=11) and 2012 (n=3) were PCR-positive with respect to APPV, whereas, all of the healthy controls (n=11) were negative. The piglets with congenital tremor sampled 2017-2018 had an odds ratio of 91.8 (95% CI 3.9128 to 2153.7842, z=2.807, P=0.0050) to test positive for APPV by qRT-PCR compared to the healthy piglets (Fishers exact test p<0.0001). These findings make it interesting to continue investigating APPV in the Swedish pig-population.ConclusionThis is the first description of atypical porcine pestivirus in piglets suffering from congenital tremor type A-II in Sweden and the Nordic countries. The virus has been present in the Swedish pig population since at least 2004.

Authors/Creators:Stenberg, Hedvig and Jacobson, Magdalena and Malmberg, Maja
Title:Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II
Year of publishing :2020
Volume:16
Article number:260
Number of Pages:8
Publisher:BMC
ISSN:1746-6148
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Congenital tremor, Type A-II, Atypical porcine pestivirus, Splay legs, Sweden, Swine, Piglets
URN:NBN:urn:nbn:se:slu:epsilon-p-107326
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107326
Additional ID:
Type of IDID
DOI10.1186/s12917-020-02445-w
Web of Science (WoS)000557708000001
ID Code:17493
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:22 Sep 2020 08:43
Metadata Last Modified:22 Sep 2020 08:43

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