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Detection and molecular characterisation of Cryptosporidium spp. in Swedish pigs

Pettersson, Emelie and Ahola, Harri and Frössling, Jenny and Wallgren, Per and Troell, Karin (2020). Detection and molecular characterisation of Cryptosporidium spp. in Swedish pigs. Acta Veterinaria Scandinavica. 62 , 40
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Abstract

Background Cryptosporidiumis a genus of apicomplexan parasites that cause enteric disease in vertebrates. In pigs, infections are most often asymptomatic, but may result in diarrhoea and poor growth. The most common species detected in pigs areC. suisandC. scrofarumwith low zoonotic potential.C. parvum, with higher zoonotic potential, may also be found. As previous knowledge on the occurrence ofCryptosporidiumin Swedish pigs is scarce, this was investigated in our study. Faecal samples from 13 pig herds were collected and a total of 222 pooled pen samples, from suckling piglets (n = 48), growers, aged 6-12 weeks (n = 57), fatteners, aged 13-24 weeks (n = 67) and adult animals (n = 50) were included. Samples were analysed using microscopy and positive samples were further analysed using polymerase chain reaction and sequencing of the 18S rRNA gene and the 28S rRNA gene to determine species. Results Cryptosporidiumspp. were detected in all sampled herds and in 25% (56/222) of the individual pen samples. Infections were most common in growers and fatteners with 51% (29/57) and 35% (20/67) positive samples in each group, respectively. The piglets had 8% (4/48) positive samples and adults had 6% (3/50). Species determination showedC. suisandC. scrofarumin piglets and growers,C. scrofarumin the fatteners, andC. suisandC. parvumin the adults. Although no mixed infections could be confirmed we saw signs of double peaks in the 28S rRNA gene chromatograms, possibly indicating more than one species present per sample. Conclusion Cryptosporidiumspp. were detected on every sampled farm and in 25% of the individual pen samples in our study. We therefore conclude thatCryptosporidiumspp. are present and likely common in Swedish pig herds, where pigs are loose and reared on solid floors. However, none of the farms reported any problems with poor weight gain, diarrhoea, or reduced appetite in their pig herds. The pig adaptedC. suisandC. scrofarumwere the predominant species identified. Two samples were positive for the more zoonoticC. parvum, and pigs should hence not be disregarded as a possible source of zoonotic cryptosporidiosis.

Authors/Creators:Pettersson, Emelie and Ahola, Harri and Frössling, Jenny and Wallgren, Per and Troell, Karin
Title:Detection and molecular characterisation of Cryptosporidium spp. in Swedish pigs
Year of publishing :2020
Volume:62
Article number:40
Number of Pages:7
Publisher:BMC
ISSN:0044-605X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:18S rRNA, 28s rRNA, Cryptosporidiosis, C parvum, C scrofarum, C suis, Genotyping, Parasite, Zoonosis
URN:NBN:urn:nbn:se:slu:epsilon-p-107342
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107342
Additional ID:
Type of IDID
DOI10.1186/s13028-020-00537-z
Web of Science (WoS)000557467900001
ID Code:17501
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:22 Sep 2020 09:24
Metadata Last Modified:22 Sep 2020 09:24

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