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Physiological, genetic and proteomic characterization of Arthrobacter chlorophenolicus during growth on different phenolic substrates or temperatures

Unell, Maria (2008). Physiological, genetic and proteomic characterization of Arthrobacter chlorophenolicus during growth on different phenolic substrates or temperatures. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2008:26
ISBN 978-91-85913-59-6
[Doctoral thesis]

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Abstract

The species Arthrobacter chlorophenolicus was isolated from soil because of its ability to degrade high concentrations of 4-chlorophenol and other toxic aromatic compounds. It is also able to grow on and degrade phenolic compounds at low temperatures as well as during extreme temperature fluctuations. In this thesis the degradation pathway through which 4-chlorophenol is degraded by this organism is described. It is an efficient and very unusual 4-chlorophenol catabolic pathway for aerobic bacteria, with hydroxyquinol as the ring-cleavage substrate. In addition, A. chlorophenolicus was studied during growth on mixtures of phenolic compounds, giving new insights into substrate preferences and mechanisms behind the sequential degradation of similar compounds. Changes in cell membrane fatty acid composition at two different temperatures when phenolic compounds were added in increasing concentrations were also studied. The results have provided more information about this adaptive response in Gram positive bacteria. In addition, the proteins expressed during the same growth conditions mentioned above were identified by shotgun proteomics, confirming the catabolic routes used by this organism during growth on phenolic compounds as well as some of its stress response mechanisms. The genome sequence of A. chlorophenolicus was sequenced and annotated, revealing secrets of adaptation and stress mechanisms as well as new findings on the genome structure and catabolic activities of A. chlorophenolicus.

Authors/Creators:Unell, Maria
Title:Physiological, genetic and proteomic characterization of Arthrobacter chlorophenolicus during growth on different phenolic substrates or temperatures
Year of publishing :April 2008
Volume:2008:26
Number of Pages:56
Papers/manuscripts:
NumberReferences
ALLI Nordin, K., M. Unell, & J. K. Jansson. (2005). Novel 4-chlorophenol degradation gene cluster and degradation route via hydroxyquinol in Arthrobacter chlorophenolicus A6. Applied and Environmental Microbiology 71, 6538-6544. II Unell, M., K. Nordin, C. Jernberg, J. Stenström & J. K. Jansson (2007). Degradation of phenol mixtures by Arthrobacter chlorophenolicus. Biodegradation. In press. III Unell, M., N. Kabelitz, J. K. Jansson & H. J. Heipieper (2007). Adaptation of the psychrotroph Arthrobacter chlorophenolicus A6 to growth temperature and the presence of phenols by changes in the anteiso/iso ratio of branched fatty acids. FEMS Microbiology Letters 266, 138-143. IV Unell, M., P. E. Abraham, M. Shaw, B. Zhang, N. VerBerkmoes & J. K. Jansson. Proteomic analysis of Arthrobacter chlorophenolicus grown on different phenol substrates and growth temperatures (manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-85913-59-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:bacteria, phenolic compounds, biodegradation, bioremediation, proteins, analytical methods
Keywords:Arthrobacter chlorophenolicus, 4-chlorophenol, 4-nitrophenol, phenol, hydroxyquinol, bioremediation, shotgun proteomics
URN:NBN:urn:nbn:se:slu:epsilon-2201
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2201
ID Code:1751
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: Maria Unell
Deposited On:25 Apr 2008 00:00
Metadata Last Modified:02 Dec 2014 10:13

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