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Microbial communities and Food safety aspects of crickets (Acheta domestica) reared under controlled conditions

Cassi, Xavier Fernandez and Söderqvist, Karin and Bakeeva, Albina and Vaga, Merko and Dicksved, Johan and Vågsholm, Ivar and Jansson, Anna and Boqvist, Sofia (2020). Microbial communities and Food safety aspects of crickets (Acheta domestica) reared under controlled conditions. Journal of insects as food and feed. 6 , 429-440
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Abstract

In an approach combining microbiological culture methods with high-throughput sequencing, this study investigated the microbial communities (bacteria, moulds and yeasts) in Swedish-produced edible crickets (Acheta domesticus) reared in a controlled environment. The effects of different feeds on microbial loads and populations in crickets were also studied. The crickets used were third-generation offspring from wild-caught individuals from Sweden, which are adapted to grow in a laboratory environment. The efficiency of rinsing to decrease microbial load was evaluated not obtaining a significant decrease of plating counts for total aerobic counts (TAC) and Enterobacteriaceae. Crickets were divided into three batches and fed different diets (control feed, early-cut red clover hay (ECH), late-cut fresh red clover (LCF)) for 62 days. Bacterial numbers (TAC and Enterobacteriaceae) on whole raw crickets ranged between 7 and 8 log cfu/g. Pre-rinsing in water did not reduce these levels (P=0.19). All batches tested negative for the food-borne bacteria Salmonella, Listeria monocytogenes, Bacillus cereus and Clostridium perfringens. The mean mould count for crickets fed control feed was 2.8 log cfu/g, while the values for crickets fed ECH and LCF were 4.2 and 4.5 log cfu/g, respectively. The dominant bacterial communities were Proteobacteria, Bacteroidetes and Firmicutes, with Firmicutes and Proteobacteria dominating in crickets fed control feed, Firmicutes dominating in crickets fed LCF and Proteobacteria dominating in crickets fed ECH. Aspergillus flavus, a fungus that is capable of producing mycotoxins, was detected in control feed and ECH reared crickets. More work is needed to identify specific food-borne pathogens in edible crickets and establish possible bacterial quality reference values, as an important step in developing microbial quality and safety parameters to ensure consumer safety.

Authors/Creators:Cassi, Xavier Fernandez and Söderqvist, Karin and Bakeeva, Albina and Vaga, Merko and Dicksved, Johan and Vågsholm, Ivar and Jansson, Anna and Boqvist, Sofia
Title:Microbial communities and Food safety aspects of crickets (Acheta domestica) reared under controlled conditions
Year of publishing :2020
Volume:6
Page range:429-440
Number of Pages:12
Publisher:Wageningen Academic Publishers
ISSN:2352-4588
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-Share Alike 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Other Veterinary Science
Keywords:insects, food safety, novel food, culture-independent methods, microbial load
URN:NBN:urn:nbn:se:slu:epsilon-p-107372
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107372
Additional ID:
Type of IDID
DOI10.3920/JIFF2019.0048
Web of Science (WoS)000562087000010
ID Code:17529
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
VH - Faculty of Veterinary Medicine and Animal Science
Department:(NL, NJ) > Department of Molecular Sciences
(VH) > Department of Biomedical Science and Veterinary Public Health
(VH) > Dept. of Anatomy, Physiology and Biochemistry
(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:05 Oct 2020 06:04
Metadata Last Modified:20 Oct 2020 10:10

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