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Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers

Juma, Ibrahim and Geleta, Mulatu Dida and Nyomora, Agnes and Saripella, Ganapathi Varma and Persson Hovmalm, Helena and Carlsson, Anders S. and Fatih, Moneim and Ortiz, Rodomiro (2020). Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers. Hereditas. 157 , 40 , 1-12
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Abstract

Background: Avocado is an important cash crop in Tanzania, however its genetic diversity is not thoroughly investigated. This study was undertaken to explore the genetic diversity of avocado in the southern highlands using microsatellite markers. A total of 226 local avocado trees originating from seeds were sampled in eight districts of the Mbeya, Njombe and Songwe regions. Each district was considered as a population. The diversity at 10 microsatellite loci was investigated. Results: A total of 167 alleles were detected across the 10 loci with an average of 16.7 ± 1.3 alleles per locus. The average expected and observed heterozygosity were 0.84 ± 0.02 and 0.65 ± 0.04, respectively. All but two loci showed a significant deviation from the Hardy-Weinberg principle. Analysis of molecular variance showed that about 6% of the variation was partitioned among the eight geographic populations. Population FST pairwise comparisons revealed lack of genetic differentiation for the seven of 28 population pairs tested. The principal components analysis (PCA) and hierarchical cluster analysis showed a mixing of avocado trees from different districts. The model-based STRUCTURE subdivided the trees samples into four major genetic clusters. Conclusion: High diversity detected in the analysed avocado germplasm implies that this germplasm is a potentially valuable source of variable alleles that might be harnessed for genetic improvement of this crop in Tanzania. The mixing of avocado trees from different districts observed in the PCA and dendrogram points to strong gene flow among the avocado populations, which led to population admixture revealed in the STRUCTURE analysis. However, there is still significant differentiation among the tree populations from different districts that can be utilized in the avocado breeding program.

Authors/Creators:Juma, Ibrahim and Geleta, Mulatu Dida and Nyomora, Agnes and Saripella, Ganapathi Varma and Persson Hovmalm, Helena and Carlsson, Anders S. and Fatih, Moneim and Ortiz, Rodomiro
Title:Genetic diversity of avocado from the southern highlands of Tanzania as revealed by microsatellite markers
Year of publishing :2020
Volume:157
Article number:40
Number of Pages:12
Publisher:BMC
ISSN:0018-0661
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Keywords:breeding, genetic admixture, germplasm management, ssr markers, population structure
URN:NBN:urn:nbn:se:slu:epsilon-p-107720
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107720
Additional ID:
Type of IDID
DOI10.1186/s41065-020-00150-0
ID Code:17539
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
(LTJ, LTV) > Plant Breeding and Biotechnology (until 121231)
Deposited By: SLUpub Connector
Deposited On:05 Oct 2020 06:27
Metadata Last Modified:05 Oct 2020 06:27

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