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Effect of Bt toxin Cry1Ab on two freshwater caddisfly shredders - an attempt to establish dose-effect relationships through food-spiking

Pott, Antonia and Bundschuh, Mirco and Schulz, Ralf (2020). Effect of Bt toxin Cry1Ab on two freshwater caddisfly shredders - an attempt to establish dose-effect relationships through food-spiking. Scientific Reports. 10 , 5262
[Journal article]

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Abstract

Genetically modified organisms (GMOs), which produce Bacillus thuringiensis (Bt) toxins, are widely used in agriculture in some parts of the world. Despite this, ecotoxicological methods, tailored to GMOs, are lacking to assess effects on aquatic environments. With the objective to investigate a food-related exposure pathway for aquatic shredders, we used a new food-spiking method while caddisfly larvae (Chaetopteryx spec., Sericostoma spec.) served as test species. Pure Cry1Ab toxins were spiked on black alder leaf discs and subsequently used in a feeding experiment. The toxin did not influence larval mortality compared to the control. The results, however, showed significant effects on larval lipid content (Chaetopteryx spec.) and development (Sericostoma spec.) at concentrations of 17.2 and 132.4 ng Cry1Ab/mg leaf, respectively. These changes are indicative for impacts on the fitness of the specimen and thus relevant in a risk assessment context. Ultimately, the food-spiking method allowed applying different Bt toxin concentrations leading to the establishment of dose-response relationships for various response variables. The use of long test durations and sublethal endpoints (consumption, lipid content, growth, larval instars) is, moreover, advisable when testing GMO effects.

Authors/Creators:Pott, Antonia and Bundschuh, Mirco and Schulz, Ralf
Title:Effect of Bt toxin Cry1Ab on two freshwater caddisfly shredders - an attempt to establish dose-effect relationships through food-spiking
Year of publishing :2020
Volume:10
Article number:5262
Number of Pages:9
ISSN:2045-2322
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
URN:NBN:urn:nbn:se:slu:epsilon-p-107853
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107853
Additional ID:
Type of IDID
DOI10.1038/s41598-020-62055-2
Web of Science (WoS)000563366200009
ID Code:17594
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:24 Sep 2020 14:00
Metadata Last Modified:24 Sep 2020 14:01

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