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Effects of season and single layer centrifugation on bull sperm quality in Thailand

Nongbua, Thanapol and Am-in, Nutthee and Suwimonteerabutr, Junpen and Johannisson, Anders and Morrell, Jane (2020). Effects of season and single layer centrifugation on bull sperm quality in Thailand. Asian-Australasian journal of animal sciences. 33 , 1411-1420
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Abstract

Objective: The aim of study was to investigate the effects of season and single layer centri-fugation (SLC) before cryopreservation on post-thaw bull sperm quality in Thailand.Methods: Semen was collected from 6 bulls (Bos indicus) in summer, rainy season and winter 2014 through 2016. Semen characteristics, sperm morphology, sperm kinematics, viability, chromatin structure and mitochondrial membrane were evaluated. Meteorological data were available from the local meteorological station;Results: Season had an effect on semen characteristics in the raw ejaculate, with higher proportions of normal spermatozoa and lower abnormalities in winter than in the other two seasons. Sperm kinematics, viability, DNA fragmentation index, and mitochondrial membrane potential were not different between seasons. Sperm samples selected by SLC had greater normal morphology and a lower proportion with bent tails than controls and higher values of progressive motility (PRO), beat cross frequency, linearity, straightness, wobble (WOB), and lower values of slow motility, velocity average path (VAP), velocity curved line, and amplitude of lateral head displacement than controls. In addition, SLC-selection had a favorable effect on PRO, VAP, and WOB that differed among seasons.Conclusion: Our results suggested that these bulls were well adapted to their location, with season having an effect on sperm morphology. Moreover, SLC could be used prior to cryo-preservation, regardless of season, to enhance normal morphology and kinematics of bull sperm samples without adversely affecting other parameters of sperm quality. However, there was considerable variation among bulls in DNA fragmentation index, mitochondrial membrane potential and sperm viability. In addition, SLC had a positive effect on sperm morphology and sperm kinematics, which could be expected to influence fertility.

Authors/Creators:Nongbua, Thanapol and Am-in, Nutthee and Suwimonteerabutr, Junpen and Johannisson, Anders and Morrell, Jane
Title:Effects of season and single layer centrifugation on bull sperm quality in Thailand
Year of publishing :2020
Volume:33
Page range:1411-1420
Number of Pages:10
ISSN:1011-2367
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Temperature, Humidity, Bovine Semen Quality, Colloid Centrifugation, Frozen Semen
URN:NBN:urn:nbn:se:slu:epsilon-p-107855
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107855
Additional ID:
Type of IDID
DOI10.5713/ajas.19.0624
Web of Science (WoS)000566458300005
ID Code:17596
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:24 Sep 2020 14:15
Metadata Last Modified:24 Sep 2020 14:21

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