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Cell-surface receptors enable perception of extracellular cytokinins

Antoniadi, Ioanna and Novak, Ondrej and Gelova, Zuzana and Johnson, Alexander and Plihal, Ondrej and Simersky, Radim and Mik, Vaclav and Vain, Thomas and Mateo-Bonmatí, Eduardo and Karady, Michal and Pernisova, Marketa and Plackova, Lenka and Opassathian, Korawit and Hejatko, Jan and Robert, Stephanie and Friml, Jiri and Dolezal, Karel and Ljung, Karin and Turnbull, Colin (2020). Cell-surface receptors enable perception of extracellular cytokinins. Nature Communications. 11 , 4284
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Abstract

Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses. The main site of cytokinin perception in plant cells is thought to be the endoplasmic reticulum where most cytokinin receptors localise. Here via the use of bioactive probes that cannot enter plant cells and super-resolution microscopy, Antoniadi et al. show that cytokinin can also be perceived at the plasma membrane.

Authors/Creators:Antoniadi, Ioanna and Novak, Ondrej and Gelova, Zuzana and Johnson, Alexander and Plihal, Ondrej and Simersky, Radim and Mik, Vaclav and Vain, Thomas and Mateo-Bonmatí, Eduardo and Karady, Michal and Pernisova, Marketa and Plackova, Lenka and Opassathian, Korawit and Hejatko, Jan and Robert, Stephanie and Friml, Jiri and Dolezal, Karel and Ljung, Karin and Turnbull, Colin
Title:Cell-surface receptors enable perception of extracellular cytokinins
Year of publishing :2020
Volume:11
Article number:4284
Number of Pages:10
ISSN:2041-1723
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-107881
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107881
Additional ID:
Type of IDID
DOI10.1038/s41467-020-17700-9
Web of Science (WoS)000567931000001
ID Code:17609
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:28 Sep 2020 09:15
Metadata Last Modified:28 Sep 2020 09:21

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