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Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum

Kubiasova, Karolina and Montesinos, Juan Carlos and Samajova, Olga and Nisler, Jaroslav and Mik, Vaclav and Semeradova, Hana and Plihalova, Lucie and Novak, Ondrej and Marhavy, Peter and Cavallari, Nicola and Zalabak, David and Berka, Karel and Galuszka, Petr and Samaj, Jozef and Strnad, Miroslav and Benkova, Eva and Plihal, Ondrej and Spichal, Lukas (2020). Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. 11 , 4285
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Abstract

Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER. Cytokinin receptors predominantly localize to the endoplasmic reticulum. Here, Kubiasova et al. use a cytokinin fluoroprobe to show that ER-localized cytokinin receptors can enter the secretory pathway, reach the plasma membrane and undergo vesicular recycling, suggesting multiple sites of cytokinin perception.

Authors/Creators:Kubiasova, Karolina and Montesinos, Juan Carlos and Samajova, Olga and Nisler, Jaroslav and Mik, Vaclav and Semeradova, Hana and Plihalova, Lucie and Novak, Ondrej and Marhavy, Peter and Cavallari, Nicola and Zalabak, David and Berka, Karel and Galuszka, Petr and Samaj, Jozef and Strnad, Miroslav and Benkova, Eva and Plihal, Ondrej and Spichal, Lukas
Title:Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum
Year of publishing :2020
Volume:11
Article number:4285
Number of Pages:11
ISSN:2041-1723
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-107882
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107882
Additional ID:
Type of IDID
DOI10.1038/s41467-020-17949-0
Web of Science (WoS)000567931000002
ID Code:17610
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:28 Sep 2020 09:30
Metadata Last Modified:28 Sep 2020 09:31

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