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Molecular Characteristics of Rhizobia Isolated from Arachis hypogaea Grown under Stress Environment

Khalid, Rabia and Zhang, Xiao Xia and Hayat, Rifat and Ahmed, Mukhtar (2020). Molecular Characteristics of Rhizobia Isolated from Arachis hypogaea Grown under Stress Environment. Sustainability. 12 , 6259
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Abstract

The phenotypic and genotypic characterization of eight rhizobial isolates obtained fromArachis hypogaeanodules grown under stress environment was performed. Isolates were screened for their ability to tolerate different abiotic stresses (high temperature (60 degrees C), salinity (1-5% (w/v) NaCl), and pH (1-12). The genomic analysis of 16S rRNA and housekeeping genes (atpD,recA, andglnII) demonstrated that native groundnut rhizobia from these stress soils are representatives of fast growers and phylogenetically related toRhizobium sp. The phenotypic characterization (generation time, carbon source utilization) also revealed the isolates as fast-growing rhizobia. All the isolates can tolerate NaCl up to 3% and were able to grow between 20 and 37 degrees C with a pH between 5 to 10, indicating that the isolates were alkali and salt-tolerant. The tested isolates effectively utilize mono and disaccharides as carbon source. Out of eight, three rhizobial isolates (BN-20, BN-23, and BN-50) were able to nodulate their host plant, exhibiting their potential to be used as native groundnut rhizobial inoculum. The plant growth promoting characterization of all isolates revealed their effectiveness to solubilize inorganic phosphate (56-290 mu g mL(-1)), synthesize indole acetic acid (IAA) (24-71 mu g mL(-1)), and amplification of nitrogen fixingnifHgene, exploring their ability to be used as groundnut biofertilizer to enhance yield and N-2-fixation for the resource poor farmers of rainfed Pothwar region.

Authors/Creators:Khalid, Rabia and Zhang, Xiao Xia and Hayat, Rifat and Ahmed, Mukhtar
Title:Molecular Characteristics of Rhizobia Isolated from Arachis hypogaea Grown under Stress Environment
Year of publishing :2020
Volume:12
Article number:6259
Number of Pages:15
ISSN:2071-1050
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Arachis hypogaea, abiotic stress, Rhizobium, 16S rRNA, housekeeping genes, phenotypic characterization, biofertilizer
URN:NBN:urn:nbn:se:slu:epsilon-p-107910
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107910
Additional ID:
Type of IDID
DOI10.3390/su12156259
Web of Science (WoS)000567285000001
ID Code:17615
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Agricultural Research for Northern Sweden
(VH) > Dept. of Agricultural Research for Northern Sweden
Deposited By: SLUpub Connector
Deposited On:28 Sep 2020 11:15
Metadata Last Modified:28 Sep 2020 11:21

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