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Cytokinins in higher plants : biosynthesis and interaction with auxins

Åstot-Lundmark, Crister (2000). Cytokinins in higher plants : biosynthesis and interaction with auxins. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae. Silvestria, 1401-6230
ISBN 91-576-5866-8
[Doctoral thesis]

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Abstract

Cytokinin biosynthesis and cytokinin/auxin interactions were studied in wildtype and hormone-overproducing plants. To enable the physiological studies to be performed, analytical tools based on liquid chromatography/ mass spectrometry (LC/MS) were developed, allowing cytokinins in minute amounts of plant tissue to be analysed.
A method for in-vivo deuterium labelling of cytokinins provided evidence for the existence of an alternative, iPAMP-independent pathway for cytokinin biosynthesis in plants. The alternative pathway was active both in wildtype plants and in plants expressing the isopentenyltransferase (ipt)-gene from Agrobacterium tumefaciens. In the ipt-expressing, cytokinin overproducing plants, the rate of ZRMP production was found to be 66 times higher than that of iPAMP formation. This indicates that iPAMP is not the major precursor of ZRMP in the bacterial pathway.
IAA biosynthesis was also studied, in tobacco, to determine the relative importance of tryptophan-dependent and tryptopan-independent biosynthesis. From tracer studies, it was concluded that the independent pathway contributed the majority of the IAA synthesised during vegetative growth.
In another series of experiments, cytokinin/auxin interactions in hormoneoverproducing plants were studied. Cytokinin overproduction was shown to decrease the rate of auxin biosynthesis, resulting in lower levels of IAA. Likewise, lower cytokinin levels were found in plants that overproduced auxins. In order to investigate the interactions further, cytokinin and auxin overproducing plants were crossed. The offspring expressed all the phenotypic traits associated with both of the parental lines, giving rise to a mixed phenotype. Surprisingly, did not the hormone levels of the cross significantly differ from wildtype plants. Thus, were the increased hormone levels of the parental lines downregulated in the cross but the plant line did still express the phenotypical traits associated with auxin- and cytokinin-overproduction

Authors/Creators:Åstot-Lundmark, Crister
Title:Cytokinins in higher plants : biosynthesis and interaction with auxins
Year of publishing :2000
Number:132
Number of Pages:50
Publisher:Swedish University of Agricultural Sciences
ISBN for printed version:91-576-5866-8
ISSN:1401-6230
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:cytokinin, auxin, biosynthesis, isopentenyltransferase, transgenic plants, Arbidopsis thaliana, Nicotiana tabacum, Agrobacterium tumefaciens, LC-MS, deuterium oxide, in-vivo labelling
URN:NBN:urn:nbn:se:slu:epsilon-p-107946
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107946
ID Code:17684
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:05 Oct 2020 10:31
Metadata Last Modified:08 Oct 2020 12:44

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