Home About Browse Search
Svenska


AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays

Kumar, Jagarlamudi Kiran and Holmgren, Sofia and Levedahl, Kerstin Hamberg and Hoglund, Martin and Venge, Per and Eriksson, Staffan (2020). AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays. Biotechniques. 68 , 335-342
[Journal article]

[img] PDF
1MB

Abstract

Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays for measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) and TK1 ELISA from Abcam. Overall, the TK 210 ELISA showed higher sensitivity than the Abcam TK1 ELISA for differentiating hematological malignancies (sensitivity of 0.77 vs 0.45) as well as for distinguishing sera of patients with solid tumors from those of apparently healthy individuals (0.61 vs 0.20). There was no significant difference in the TK1 protein levels determined with the TK 210 ELISA between different age groups from apparently healthy individuals. These results strongly indicate that the AroCell TK 210 ELISA is accurate and sensitive enough to be a valuable tool in cancer management.METHOD SUMMARYThymidine kinase 1 (TK1) is an enzyme that leaks from S phase cells as a result of high cell turnover. Commercially available TK activity assays have certain limitations; to overcome these, we developed a dual monoclonal antibody-based ELISA, the AroCell TK 210 ELISA, which is commercially available. The ELISA includes a preincubation procedure with a special buffer that reduces high molecular weight complexes of serum TK1 and exposes the TK1 epitope to facilitate antibody binding. This provides a robust and convenient assay for the determination of TK1 protein concentrations in sera from patients with different malignancies.

Authors/Creators:Kumar, Jagarlamudi Kiran and Holmgren, Sofia and Levedahl, Kerstin Hamberg and Hoglund, Martin and Venge, Per and Eriksson, Staffan
Title:AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays
Year of publishing :2020
Volume:68
Page range:335-342
Number of Pages:8
ISSN:0736-6205
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:human TK1 antibodies, AroCell TK 210 ELISA, hematological malignancies, serum thymidine kinase 1 (STK1)
URN:NBN:urn:nbn:se:slu:epsilon-p-108017
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108017
Additional ID:
Type of IDID
DOI10.2144/btn-2019-0148
Web of Science (WoS)000568027400009
ID Code:17780
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:12 Oct 2020 13:48
Metadata Last Modified:12 Oct 2020 13:51

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits