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Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s

Pang, Yanhong and Kovachev, Petar and Sanyal, Suparna (2020). Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s. International Journal of Molecular Sciences. 21 , 6340
[Research article]

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The role of the nucleic acids in prion aggregation/disaggregation is becoming more and more evident. Here, using HET-s prion from fungiPodospora anserina(P. anserina) as a model system, we studied the role of RNA, particularly of different domains of the ribosomal RNA (rRNA), in its aggregation process. Our results using Rayleigh light scattering, Thioflavin T (ThT) binding, transmission electron microscopy (TEM) and cross-seeding assay show that rRNA, in particular the domain V of the major rRNA from the large subunit of the ribosome, substantially prevents insoluble amyloid and amorphous aggregation of the HET-s prion in a concentration-dependent manner. Instead, it facilitates the formation of the soluble oligomeric "seeds", which are capable of promoting de novo HET-s aggregation. The sites of interactions of the HET-s prion protein on domain V rRNA were identified by primer extension analysis followed by UV-crosslinking, which overlap with the sites previously identified for the protein-folding activity of the ribosome (PFAR). This study clarifies a missing link between the rRNA-based PFAR and the mode of propagation of the fungal prions.

Authors/Creators:Pang, Yanhong and Kovachev, Petar and Sanyal, Suparna
Title:Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s
Series Name/Journal:International Journal of Molecular Sciences
Year of publishing :2020
Article number:6340
Number of Pages:14
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:ribosomal RNA, prion aggregation, P, anserinaprion protein HET-s, PFAR
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Additional ID:
Type of IDID
Web of Science (WoS)000569669000001
ID Code:17821
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:14 Oct 2020 15:40
Metadata Last Modified:15 Jan 2021 19:45

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