Pang, Yanhong and Kovachev, Petar and Sanyal, Suparna
(2020).
Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s.
International Journal of Molecular Sciences. 21
, 6340
[Research article]
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Abstract
The role of the nucleic acids in prion aggregation/disaggregation is becoming more and more evident. Here, using HET-s prion from fungiPodospora anserina(P. anserina) as a model system, we studied the role of RNA, particularly of different domains of the ribosomal RNA (rRNA), in its aggregation process. Our results using Rayleigh light scattering, Thioflavin T (ThT) binding, transmission electron microscopy (TEM) and cross-seeding assay show that rRNA, in particular the domain V of the major rRNA from the large subunit of the ribosome, substantially prevents insoluble amyloid and amorphous aggregation of the HET-s prion in a concentration-dependent manner. Instead, it facilitates the formation of the soluble oligomeric "seeds", which are capable of promoting de novo HET-s aggregation. The sites of interactions of the HET-s prion protein on domain V rRNA were identified by primer extension analysis followed by UV-crosslinking, which overlap with the sites previously identified for the protein-folding activity of the ribosome (PFAR). This study clarifies a missing link between the rRNA-based PFAR and the mode of propagation of the fungal prions.
Authors/Creators: | Pang, Yanhong and Kovachev, Petar and Sanyal, Suparna | ||||||
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Title: | Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s | ||||||
Series Name/Journal: | International Journal of Molecular Sciences | ||||||
Year of publishing : | 2020 | ||||||
Volume: | 21 | ||||||
Article number: | 6340 | ||||||
Number of Pages: | 14 | ||||||
ISSN: | 1661-6596 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology | ||||||
Keywords: | ribosomal RNA, prion aggregation, P, anserinaprion protein HET-s, PFAR | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-108111 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108111 | ||||||
Additional ID: |
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ID Code: | 17821 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Department of Molecular Sciences | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 14 Oct 2020 15:40 | ||||||
Metadata Last Modified: | 15 Jan 2021 19:45 |
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