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Monitoring the control methods of Heterobasidion annosum s.l. root rot

Samils, Nicklas (2008). Monitoring the control methods of Heterobasidion annosum s.l. root rot. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2008:47
ISBN 978-91-85913-80-0
[Doctoral thesis]

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Abstract

This thesis summarises the author's work on the effectiveness of two control methods against the necrotrophic fungal pathogen Heterobasidion annosum s.l. (Fr.) Bref. The studied control methods were stump treatments through the application of a fungal biocontrol agent (Phlebiopsis gigantea) and application of urea solution. An additional component to this thesis was to develop a useful Agrobacterium tumefaciens-mediated transformation system for the H. annosum s.l. The potential risk for a build-up in the capacity of H. annosum s.l. to resist overgrowth by P. gigantea was investigated by utilising a P. gigantea strain used in the commercial preparation Rotstop and different strains of H. annosum s.l. The impact on intraspecific genetic diversity from the P. gigantea used in Rotstop four years after stump treatment was estimated using both microsatellite markers and AP-PCR. The long-term effect of urea treatment during first rotation thinning of Picea abies stands was evaluated 15 years post-treatment. An A. tumefaciens-mediated transformation system was developed using two different transformation plasmids and a North American H. annosum strain. The results revealed differences between H. annosum s.l. strains in their ability to resist overgrowth by P. gigantea. This trait was found to be heritable which suggests that H. annosum s.l. has the capacity to develop more tolerant strains against the biocontrol agent if the conditions are met. Also, one QTL effect associated with this trait was identified. Genetic diversity was lower within Rotstop treated plots and the resident population was more genetically similar to the applied P. gigantea strain than other populations sampled in Sweden. This effect on genetic structure was not detected 100-200 m from the treated plot which suggests that stump treatments with P. gigantea only has a local effect on genetic diversity. Urea treated plots exhibited less rot than untreated plots and the urea treatments also significantly reduced the occurrence of windthrown trees. This shows that urea treatment during thinning operations is an efficient method to reduce root rot in P. abies stands. A total of 18 H. annosum s.l. transformants were obtained from the A. tumefaciens-mediated transformation system. The transformants expressed GFP throughout the mycelia and had a normal growth rate. This system will be very useful for genomic work and interaction studies.

Authors/Creators:Samils, Nicklas
Title:Monitoring the control methods of Heterobasidion annosum s.l. root rot
Year of publishing :2008
Volume:2008:47
Number of Pages:56
Papers/manuscripts:
NumberReferences
ALLI. Samils, N., Olson, Å. & Stenlid, J. 2008. The capacity in Heterobasidion annosum s.l. to resist overgrowth by the biocontrol agent Phlebiopsis gigantea is a heritable trait. Biological Control 45, 419-426. II. Samils, N., Vasaitis, R. & Stenlid J. Impact of the biological control agent Phlebiopsis gigantea on genetic structure of resident populations in the Baltic Sea region. Manuscript. III. Oliva, J., Samils, N., Johansson, U., Bendz-Hellgren, M. & Stenlid, J. 2008. Urea treatment reduced Heterobasidion annosum s.l. root rot in Picea abies after 15 years. Forest Ecology and Management 255, 2876-2882. IV. Samils, N., Elfstrand, M., Lindner-Czederpiltz, D., L., Fahleson, J., Olson, Å., Dixelius, C. & Stenlid, J. 2006. Development of a rapid and simple Agrobacterium tumefaciens-mediated transformation system for the fungal pathogen Heterobasidion annosum. FEMS Microbiology Letters 255, 82-88.
Place of Publication:Uppsala
ISBN for printed version:978-91-85913-80-0
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:heterobasidion annosum, pathogens, root rots, fungal diseases, disease control, biological control, phlebiopsis gigantea, urea, heritability, agrobacterium tumefaciens, picea abies, pinus sylvestris
Keywords:Heterobasidion annosum, Phlebiopsis gigantea, Rotstop, biological control, biocontrol, urea, heritability, GFP, Agrobacterium tumefaciens
URN:NBN:urn:nbn:se:slu:epsilon-2448
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2448
ID Code:1790
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: Nicklas Samils
Deposited On:19 Aug 2008 00:00
Metadata Last Modified:02 Dec 2014 10:14

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