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Quantitative proteomics and transcriptomics of potato in response to Phytophthora infestans in compatible and incompatible interactions

UNSPECIFIED (2014). Quantitative proteomics and transcriptomics of potato in response to Phytophthora infestans in compatible and incompatible interactions. BMC Genomics. 15 , 497
[Journal article]

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Abstract

Background: In order to get global molecular understanding of one of the most important crop diseases worldwide, we investigated compatible and incompatible interactions between Phytophthora infestans and potato (Solanum tuberosum). We used the two most field-resistant potato clones under Swedish growing conditions, which have the greatest known local diversity of P. infestans populations, and a reference compatible cultivar.Results: Quantitative label-free proteomics of 51 apoplastic secretome samples (PXD000435) in combination with genome-wide transcript analysis by 42 microarrays (E-MTAB-1515) were used to capture changes in protein abundance and gene expression at 6, 24 and 72 hours after inoculation with P. infestans. To aid mass spectrometry analysis we generated cultivar-specific RNA-seq data (E-MTAB-1712), which increased peptide identifications by 17%. Components induced only during incompatible interactions, which are candidates for hypersensitive response initiation, include a Kunitz-like protease inhibitor, transcription factors and an RCR3-like protein. More secreted proteins had lower abundance in the compatible interaction compared to the incompatible interactions. Based on this observation and because the well-characterized effector-target C14 protease follows this pattern, we suggest 40 putative effector targets.Conclusions: In summary, over 17000 transcripts and 1000 secreted proteins changed in abundance in at least one time point, illustrating the dynamics of plant responses to a hemibiotroph. Half of the differentially abundant proteins showed a corresponding change at the transcript level. Many putative hypersensitive and effector-target proteins were single representatives of large gene families.

Authors/Creators:UNSPECIFIED
Title:Quantitative proteomics and transcriptomics of potato in response to Phytophthora infestans in compatible and incompatible interactions
Year of publishing :2014
Volume:15
Article number:497
Number of Pages:18
ISSN:1471-2164
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:None
URN:NBN:urn:nbn:se:slu:epsilon-p-106699
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106699
Additional ID:
Type of IDID
DOI10.1186/1471-2164-15-497
Web of Science (WoS)000338248100003
ID Code:17940
Deposited By: SLUpub Connector
Deposited On:27 Oct 2020 11:11
Metadata Last Modified:27 Oct 2020 11:21

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