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Extenders for alpaca epididymal spermatozoa: Comparison of INRA96 and andromed

Al-Essawe, Essraa M and Abraham, Maria Celina and Kunkitti, Panisara and Axner, Eva and de Verdier, Kerstin and Båge, Renee and Morrell, Jane (2020). Extenders for alpaca epididymal spermatozoa: Comparison of INRA96 and andromed. Animal Reproduction Science. 223 , 106629
[Journal article]

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Abstract

Artificial insemination would be a useful technique for alpaca breeders to use as an aid to breeding to increase fleece quality. The technique, however, is not well developed in alpacas, partly because of the viscous nature of their seminal plasma. Castration conducted for husbandry purposes can provide a source of epididymal spermatozoa to test semen extenders or handling regimens, thus circumventing the problem of the viscous ejaculate. In this experiment, two semen extenders (Andromed and INRA96) developed for other species (bovine and equine, respectively) were tested with alpaca spermatozoa derived from the cauda epididymis. Sperm total motility (mean ± SEM A: 29.1 ± 4.8 % compared with I: 35.4 ± 4.8 %; NS), membrane integrity (A: 58 ± 9% compared with I: 56 ± 9%; NS) and acrosome integrity (A: 65 ± 7% compared with I: 54 ± 7%; NS) were not different between the two extenders. Progressive motility with use of INRA96 was greater after incubating for 30 min than after incubating for 10 min (35 ± 4% vs. 12 ± 4%, respectively; P = 0.03). In conclusion, viable epididymal spermatozoa could be extracted from the castrated organs after overnight transport. There were no differences in sperm quality between the two extenders; therefore, it appears that either extender could be used for alpaca spermatozoa. These results could help in the development of a technique for artificial insemination in alpacas.

Authors/Creators:Al-Essawe, Essraa M and Abraham, Maria Celina and Kunkitti, Panisara and Axner, Eva and de Verdier, Kerstin and Båge, Renee and Morrell, Jane
Title:Extenders for alpaca epididymal spermatozoa: Comparison of INRA96 and andromed
Year of publishing :2020
Volume:223
Article number:106629
Number of Pages:5
ISSN:0378-4320
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Vicugna pacos, camelid, sperm handling, sperm quality
URN:NBN:urn:nbn:se:slu:epsilon-p-108267
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108267
Additional ID:
Type of IDID
DOI10.1016/j.anireprosci.2020.106629
ID Code:17965
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:28 Oct 2020 07:23
Metadata Last Modified:28 Oct 2020 07:31

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