Home About Browse Search
Svenska


RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa

Prakash, Mani Arul and Kumaresan, Arumugam and Sinha, Manish Kumar and Kamaraj, Elango and Mohanty, Tushar Kumar and Datta, Tirtha Kumar and Morrell, Jane (2020). RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa. Animal Reproduction Science. 222 , 106621
[Journal article]

[img] PDF
7MB

Abstract

Sperm, which are believed to be transcriptionally and translationally inactive, synthesize RNA and proteins before there is gradual disappearance of the ribosome during chromatin compaction. Sperm transfer several functionally relevant transcripts to the oocyte, controlling maternal-zygotic transition and embryonic development. The present study was undertaken to profile and analyze sperm transcripts comprehensively using Next Generation Ribonucleic acid sequencing technology in Holstein Friesian x Tharparkar crossbred bulls. The results from global transcriptomic profiling revealed transcripts for 13,814 genes; of which 431 transcripts were expressed with >1 FPKM and 13,383 transcripts were expressed with >0 or <1 FPKM. The abundant mRNA transcripts of crossbred bull sperm were PRM1 and HMGB4. Gene ontology of transcripts with>1 FPKM revealed there was a major involvement in the structural constituent of ribosomes and translation. Results from pathway enrichment indicated the connection between ribosome, oxidative phosphorylation and spliceosome pathways and the transcripts of crossbred bull spermatozoa. The transcriptional abundance of selected genes, validated using RT-qPCR, indicated significant variations between bulls. Collectively, it may be inferred that the transcripts in crossbred bull sperm were heavily implicated in functions such as the structural constituent of ribosomes and translation, and pathways such as ribosome, oxidative phosphorylation and spliceosome. Further studies using larger sample sizes are required to understand the possible implications of transcriptomic variations on semen quality and fertility.

Authors/Creators:Prakash, Mani Arul and Kumaresan, Arumugam and Sinha, Manish Kumar and Kamaraj, Elango and Mohanty, Tushar Kumar and Datta, Tirtha Kumar and Morrell, Jane
Title:RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa
Year of publishing :2020
Volume:222
Article number:106621
Number of Pages:13
ISSN:0378-4320
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:Crossbred Bull, Spermatozoa, Transcriptomic profiling, RNA-Seq, Ribosome, Oxidative phosphorylation
URN:NBN:urn:nbn:se:slu:epsilon-p-108262
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108262
Additional ID:
Type of IDID
DOI10.1016/j.anireprosci.2020.106621
ID Code:18025
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:02 Nov 2020 09:03
Metadata Last Modified:02 Nov 2020 09:11

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits