Prakash, Mani Arul and Kumaresan, Arumugam and Sinha, Manish Kumar and Kamaraj, Elango and Mohanty, Tushar Kumar and Datta, Tirtha Kumar and Morrell, Jane
(2020).
RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa.
Animal Reproduction Science. 222
, 106621
[Journal article]
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Abstract
Sperm, which are believed to be transcriptionally and translationally inactive, synthesize RNA and proteins before there is gradual disappearance of the ribosome during chromatin compaction. Sperm transfer several functionally relevant transcripts to the oocyte, controlling maternal-zygotic transition and embryonic development. The present study was undertaken to profile and analyze sperm transcripts comprehensively using Next Generation Ribonucleic acid sequencing technology in Holstein Friesian x Tharparkar crossbred bulls. The results from global transcriptomic profiling revealed transcripts for 13,814 genes; of which 431 transcripts were expressed with >1 FPKM and 13,383 transcripts were expressed with >0 or <1 FPKM. The abundant mRNA transcripts of crossbred bull sperm were PRM1 and HMGB4. Gene ontology of transcripts with>1 FPKM revealed there was a major involvement in the structural constituent of ribosomes and translation. Results from pathway enrichment indicated the connection between ribosome, oxidative phosphorylation and spliceosome pathways and the transcripts of crossbred bull spermatozoa. The transcriptional abundance of selected genes, validated using RT-qPCR, indicated significant variations between bulls. Collectively, it may be inferred that the transcripts in crossbred bull sperm were heavily implicated in functions such as the structural constituent of ribosomes and translation, and pathways such as ribosome, oxidative phosphorylation and spliceosome. Further studies using larger sample sizes are required to understand the possible implications of transcriptomic variations on semen quality and fertility.
Authors/Creators: | Prakash, Mani Arul and Kumaresan, Arumugam and Sinha, Manish Kumar and Kamaraj, Elango and Mohanty, Tushar Kumar and Datta, Tirtha Kumar and Morrell, Jane | ||||
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Title: | RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs in crossbred bull spermatozoa | ||||
Series Name/Journal: | Animal Reproduction Science | ||||
Year of publishing : | 2020 | ||||
Volume: | 222 | ||||
Article number: | 106621 | ||||
Number of Pages: | 13 | ||||
ISSN: | 0378-4320 | ||||
Language: | English | ||||
Publication Type: | Journal article | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science. | ||||
Keywords: | Crossbred Bull, Spermatozoa, Transcriptomic profiling, RNA-Seq, Ribosome, Oxidative phosphorylation | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-108262 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108262 | ||||
Additional ID: |
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ID Code: | 18025 | ||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||
Department: | (VH) > Dept. of Clinical Sciences | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 02 Nov 2020 09:03 | ||||
Metadata Last Modified: | 15 Jan 2021 19:45 |
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