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Assessment of the F200Y mutation frequency in the beta tubulin gene of Haemonchus contortus following the exposure to a discriminating concentration of thiabendazole in the egg hatch test

Baltrusis, Paulius and Komaromyova, Michaela and Varady, Marian and von Samson-Himmelstjerna, Georg and Höglund, Johan (2020). Assessment of the F200Y mutation frequency in the beta tubulin gene of Haemonchus contortus following the exposure to a discriminating concentration of thiabendazole in the egg hatch test. Experimental Parasitology. 217 , 107957
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Abstract

The ruminant livestock production sector is under threat due to the infections with gastrointestinal nematode parasites and the subsequent development of anthelmintic resistance. One of most common and pathogenic species in small ruminants is Haemonchus contortus. The ability to control the infections with this and other gastrointestinal nematodes relies heavily on the use of anthelmintic drugs. Although resistance to all major classes of anthelmintics has been shown in H. contortus, the precise mechanism of resistance acquisition is only known for benzimidazoles. F200Y (TAC) is a common point mutation in the isotype 1 beta tubulin gene which is associated with an effective increase in the resistance towards benzimidazole drugs. Here, we show the utility of using this mutation as a marker in a droplet digital PCR assay to track how two H. contortus laboratory strains, characterized by different resistance levels, change with respect to this mutation, when subjected to increasing concentrations of thiabendazole. Additionally, we wanted to investigate whether exposure to a discriminating dose of thiabendazole in the egg hatch test resulted in the death of all H. contortus eggs with a susceptible genotype. We found the MHco5 strain to maintain an overall higher frequency of the F200Y mutation (80-100%) over all drug concentrations, whilst a steady, gradual increase from around 30%-60% was observed in the case of the MHco4 strain. This is further supported by the dose-response curves, displaying a much higher tolerance of the MHco5 strain (LD50 = 0.38 mu g/ml) in comparison to the MHco4 strain (LD50 = 0.07 mu g/ml) to the effects of thiabendazole. All things considered, we show that the F200Y mutation is still a viable and reliable marker for the detection and surveillance of benzimidazole drug resistance in H. contortus in Europe.

Authors/Creators:Baltrusis, Paulius and Komaromyova, Michaela and Varady, Marian and von Samson-Himmelstjerna, Georg and Höglund, Johan
Title:Assessment of the F200Y mutation frequency in the beta tubulin gene of Haemonchus contortus following the exposure to a discriminating concentration of thiabendazole in the egg hatch test
Year of publishing :2020
Volume:217
Article number:107957
Number of Pages:5
Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN:0014-4894
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Droplet digital PCR, Haemonchus contortus, beta tubulin, F200Y, Anthelmintic resistance, Benzimidazole
URN:NBN:urn:nbn:se:slu:epsilon-p-108379
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108379
Additional ID:
Type of IDID
DOI10.1016/j.exppara.2020.107957
Web of Science (WoS)000576359000015
ID Code:18069
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:04 Nov 2020 10:23
Metadata Last Modified:04 Nov 2020 10:31

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