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Detection and Genetic Characterization of Viruses Present in Free-Ranging Snow Leopards Using Next-Generation Sequencing

Johansson, Örjan and Ullman, Karin and Lkhagvajav, Purevjav and Wiseman, Marc and Malmsten, Jonas and Leijon, Mikael (2020). Detection and Genetic Characterization of Viruses Present in Free-Ranging Snow Leopards Using Next-Generation Sequencing. Frontiers in Veterinary Science. 7 , 645
[Journal article]

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Abstract

Snow leopards inhabit the cold, arid environments of the high mountains of South and Central Asia. These living conditions likely affect the abundance and composition of microbes with the capacity to infect these animals. It is important to investigate the microbes that snow leopards are exposed to detect infectious disease threats and define a baseline for future changes that may impact the health of this endangered felid. In this work, next-generation sequencing is used to investigate the fecal (and in a few cases serum) virome of seven snow leopards from the Tost Mountains of Mongolia. The viral species to which the greatest number of sequences reads showed high similarity was rotavirus. Excluding one animal with overall very few sequence reads, four of six animals (67%) displayed evidence of rotavirus infection. A serum sample of a male and a rectal swab of a female snow leopard produced sequence reads identical or closely similar to felid herpesvirus 1, providing the first evidence that this virus infects snow leopards. In addition, the rectal swab from the same female also displayed sequence reads most similar to feline papillomavirus 2, which is the first evidence for this virus infecting snow leopards. The rectal swabs from all animals also showed evidence for the presence of small circular DNA viruses, predominantly Circular Rep-Encoding Single-Stranded (CRESS) DNA viruses and in one case feline anellovirus. Several of the viruses implicated in the present study could affect the health of snow leopards. In animals which are under environmental stress, for example, young dispersing individuals and lactating females, health issues may be exacerbated by latent virus infections.

Authors/Creators:Johansson, Örjan and Ullman, Karin and Lkhagvajav, Purevjav and Wiseman, Marc and Malmsten, Jonas and Leijon, Mikael
Title:Detection and Genetic Characterization of Viruses Present in Free-Ranging Snow Leopards Using Next-Generation Sequencing
Year of publishing :2020
Volume:7
Article number:645
Number of Pages:9
Publisher:FRONTIERS MEDIA SA
ISSN:2297-1769
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
Keywords:snow leopard, free-ranging, virome, Mongolia, rectal swabs, next-generating sequencing, Panthera unica
URN:NBN:urn:nbn:se:slu:epsilon-p-108388
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108388
Additional ID:
Type of IDID
DOI10.3389/fvets.2020.00645
Web of Science (WoS)000575786300001
ID Code:18073
Faculty:S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology

(S) > Dept. of Wildlife, Fish and Environmental Studies
Deposited By: SLUpub Connector
Deposited On:04 Nov 2020 15:23
Metadata Last Modified:04 Nov 2020 15:31

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