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Community ecology of denitrifying bacteria in arable land

Enwall, Karin (2008). Community ecology of denitrifying bacteria in arable land. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2008:58
ISBN 978-91-85913-91-6
[Doctoral thesis]

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Abstract

Denitrifying bacteria comprise a functional guild that under anaerobic conditions is able to use nitrogenous compound for respiration. Agricultural soils are often fertilized with nitrogen, and the reduction of nitrate and nitrite to nitric oxide, nitrous oxide and dinitrogen via the denitrification pathway, leads to gaseous emissions from the nitrogenous compounds, a subject of great environmental and economic concern. This thesis examined denitrifiers, nitrate reducers, ammonia oxidizers and total bacteria, in terms of community structure, abundance and function in different arable soils. Denitrifying bacteria were described using the signature genes that encode the analytic subunits of denitrification enzymes. Appropriate targets and methods for this were evaluated. An assessment was carried out of how these biological factors are affected by different fertilizer and management practices. The spatial distribution of the denitrifying bacteria, and how this was correlated to soil properties, was also explored. Long-term application of different organic and mineral fertilizers altered both the community structure and function of the different bacterial groups. However, fertilization practices producing the most different community structure were not correlated to the treatments with the most different activities. Thus activities were not coupled to community composition. However, a study with different carbon additions, with and without nitrogen additions, showed that nitrogen addition increased the microbial activity and biomass, and in this case community composition was correlated with potential activity. In long-term fertilization, pH, C:N ratio and organic carbon content were important drivers shaping the community composition of the denitrifiers and total bacteria. Determination of the structure and size of denitrifying bacteria at field scale revealed significant relationships between denitrifying community composition, size and activity, and soil factors. These results are important in identifying the resource-based niches for denitrifiers at scales relevant for developing sustainable land management and agricultural practices.

Authors/Creators:Enwall, Karin
Title:Community ecology of denitrifying bacteria in arable land
Year of publishing :2008
Volume:2008:58
Number of Pages:65
Papers/manuscripts:
NumberReferences
ALLI Throbäck, I.N., Enwall, K., Jarvis, Å. and Hallin, S. (2004). Reassessing PCR primers targeting nirK, nirS and nosZ genes for molecular diversity surveys of denitrifying bacteria, and the analysis of community structure with DGGE. FEMS Microbiology Ecology 49(3), 401-417. II Enwall, K., Philippot, L. and Hallin, S. (2005). Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization. Applied and Environmental Microbiology 71(12), 8335-8343. III Enwall, K., Nyberg, K., Bertilsson, S., Cederlund, H., Stenström, J. and Hallin, S. (2006). Long-term impact of fertilization on activity and composition of bacterial communities and metabolic guilds in agricultural soil. Soil Biology and Biochemistry 39(1), 401-417. IV Enwall, K. and Hallin, S. Comparison of T-RFLP and DGGE techniques to assess denitrifier community composition in soil (submitted). V Wessén, G., Enwall, K., Jansson, J. and Hallin, S. Soil carbon sequestration and depletion structure bacterial communities and alter soil respiration (manuscript). VI Enwall, K., Throbäck, I.N., Stenberg, M., Söderström, M. and Hallin, S. Soil-based resources influence field-scale spatial patterns of N-cycling bacterial communities: A case study of denitrifiers (manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-85913-91-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:bacteria, denitrification, agricultural soils, soil microorganisms, microbial ecology, soil biology, fertilizer application, nitrogen cycle, electrophoresis, rflp
Keywords:agricultural soil, community structure, denitrifying bacteria, DGGE, fertilizer, long-term, microbial ecology, N cycle, T-RFLP
URN:NBN:urn:nbn:se:slu:epsilon-2525
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2525
ID Code:1825
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: Karin Enwall
Deposited On:25 Sep 2008 00:00
Metadata Last Modified:02 Dec 2014 10:14

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