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Microbial community dynamics in the phyllosphere of leafy vegetables

Darlison, Julia (2020). Microbial community dynamics in the phyllosphere of leafy vegetables. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880
ISBN 978-91-7760-650-5
eISBN 978-91-7760-651-2
[Doctoral thesis]

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Microbes interact with the phyllosphere creating a plant microbiota holobiont. From a food safety aspect the phyllosphere is especially important in leafy vegetables which are generally consumed raw. Plant-microbe interactions are complex, but well-known ecological concepts can be applied to explain the dynamics.
The microbial communities in the phyllosphere of spinach, rocket and Swiss chard were studied in field and greenhouse studies in this thesis. We examined the impact of nitrogen fertiliser application to plants on the microbiota in the phyllosphere. The influence of nitrogen fertiliser on establishment of an invading human pathogen, Escherichia coli O157:H7 gfp+ and the role of the plant species and plant cultivar were investigated. We explored the succession of microbial communities on leafy vegetables. To identify the microbial communities, culture-independent methods (Illumina MiSeq after DNA extraction from the leaf surface) and culture dependent methods (viable count and identification of bacterial isolates with 16S rRNA sequencing) were used in combination with plant physiological measurements and weather monitoring.
Plant species, environmental factors and annual variations had selective effects on the microbial communities in the phyllosphere. Increasing nitrogen supply reduced microbial diversity, as did harvesting the leaves. The concept of prevention of invasion by high diversity was not confirmed for bacterial communities in the phyllosphere. Phyllosphere microorganisms are also of interest for possibly developing new efficient and sustainable methods in agriculture and horticulture, however, before microbiomes can be exploited, more knowledge is needed about how plants and microbes interact with the environment, management and post-harvest procedures.

Authors/Creators:Darlison, Julia
Title:Microbial community dynamics in the phyllosphere of leafy vegetables
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2020
Number of Pages:92
Publisher:Department of Biosystems and Technology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-650-5
ISBN for electronic version:978-91-7760-651-2
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Keywords:phyllosphere, microbiome, microbial community, metagenomics, food safety, E. coli O157:H7 gfp+, leafy vegetables, nitrogen fertiliser, ecology concepts
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ID Code:18714
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Biosystems and Technology (from 130101)
Deposited By: SLUpub Connector
Deposited On:19 Nov 2020 15:43
Metadata Last Modified:15 Jan 2021 19:19

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