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Evaluation of the reliability of indicators of consciousness during CO2 stunning of rainbow trout and the effects of temperature

Bowman, Jennifer and van Nuland, Nicole and Hjelmstedt, Per and Berg, Lotta and Gräns, Albin (2020). Evaluation of the reliability of indicators of consciousness during CO2 stunning of rainbow trout and the effects of temperature. Aquaculture Research. 51 , 5194-5202
[Journal article]

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Abstract

A two-part experiment was conducted to determine whether visual indicators of consciousness such as equilibrium, eye-roll reflex and ventilation are reliable for evaluating whether CO2 stunning of rainbow trout (Oncorhynchus mykiss) is humane. In part 1, the time taken until the loss of visual indicators in rainbow trout during CO2 stunning was monitored under field conditions at 14, 8 and 2 degrees C. Here, we clearly demonstrate that it takes longer for visual indicators to disappear as temperature decreases, with significant differences in the time taken until the loss of equilibrium between 2 and 14 degrees C, and significant differences between all temperatures in the time taken until the loss of eye-roll reflex and ventilation. In part 2, rainbow trout were equipped with external non-invasive electrodes for recording EEG prior to, and following, CO2 stunning to assess the presence or absence of visually evoked responses (VERs), which are indicative of brain function and sensibility. The resulting EEG recordings during CO2 stunning at 10 degrees C demonstrated a poor relationship between visual indicators of consciousness and loss of sensibility, as VERs were present up to 3.5 min after ventilation was lost and up to 6.5 min after the fish lost equilibrium. Collectively, these results show that cold-water temperatures prolong the time taken until loss of consciousness and that visual indicators are insufficient for determining when sensibility is lost in rainbow trout during CO2 stunning.

Authors/Creators:Bowman, Jennifer and van Nuland, Nicole and Hjelmstedt, Per and Berg, Lotta and Gräns, Albin
Title:Evaluation of the reliability of indicators of consciousness during CO2 stunning of rainbow trout and the effects of temperature
Year of publishing :2020
Volume:51
Page range:5194-5202
Number of Pages:9
Publisher:TAYLOR & FRANCIS LTD
ISSN:1355-557X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
Keywords:brain function, electroencephalography, sensibility, unconsciousness, welfare
URN:NBN:urn:nbn:se:slu:epsilon-p-107835
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-107835
Additional ID:
Type of IDID
DOI10.1111/are.14857
Web of Science (WoS)000564169500001
ID Code:18739
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Environment and Health
Deposited By: SLUpub Connector
Deposited On:25 Nov 2020 10:23
Metadata Last Modified:15 Jan 2021 19:19

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