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Polycomb Repressive Complex 2-mediated histone modification H3K27me3 is associated with embryogenic potential in Norway

Nakamura, Miyuki and Batista, Rita and Köhler, Claudia and Hennig, Lars (2020). Polycomb Repressive Complex 2-mediated histone modification H3K27me3 is associated with embryogenic potential in Norway. Journal of Experimental Botany. 71 , 6366-6378
[Journal article]

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Abstract

Epigenetic reprogramming during germ cell formation is essential to gain pluripotency and thus embryogenic potential. The histone modification H3K27me3, which is catalysed by the Polycomb repressive complex 2 (PRC2), regulates important developmental processes in both plants and animals, and defects in PRC2 components cause pleiotropic developmental abnormalities. Nevertheless, the role of H3K27me3 in determining embryogenic potential in gymnosperms is still elusive. To address this, we generated H3K27me3 profiles of Norway spruce (Picea abies) embryonic callus and non-embryogenic callus using CUT&RUN, which is a powerful method for chromatin profiling. Here, we show that H3K27me3 mainly accumulated in genic regions in the Norway spruce genome, similarly to what is observed in other plant species. Interestingly, H3K27me3 levels in embryonic callus were much lower than those in the other examined tissues, but markedly increased upon embryo induction. These results show that H3K27me3 levels are associated with the embryogenic potential of a given tissue, and that the early phase of somatic embryogenesis is accompanied by changes in H3K27me3 levels. Thus, our study provides novel insights into the role of this epigenetic mark in spruce embryogenesis and reinforces the importance of PRC2 as a key regulator of cell fate determination across different plant species.

Authors/Creators:Nakamura, Miyuki and Batista, Rita and Köhler, Claudia and Hennig, Lars
Title:Polycomb Repressive Complex 2-mediated histone modification H3K27me3 is associated with embryogenic potential in Norway
Year of publishing :2020
Volume:71
Page range:6366-6378
Number of Pages:13
ISSN:0022-0957
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:embryogenic callus, gymnosperm, H3K27me3, histone modification, Norway spruce, somatic embryos
URN:NBN:urn:nbn:se:slu:epsilon-p-108795
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108795
Additional ID:
Type of IDID
DOI10.1093/jxb/eraa365
ID Code:18744
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:25 Nov 2020 15:23
Metadata Last Modified:15 Jan 2021 19:44

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