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Implementation of molecular detection techniques in the field of veterinary virology

with special reference to the ligation-based methodologies

Gyarmati, Péter (2008). Implementation of molecular detection techniques in the field of veterinary virology. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2008:83
ISBN 978-91-86195-16-8
[Doctoral thesis]

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Abstract

This thesis deals with the molecular diagnosis of infectious diseases concerning animal and human health: in particular, with diseases notifiable to the World Organization for Animal Health, OIE (the vesicular complex and avian influenza), as well as with Hepatitis E, representing emerging zoonotic aspects. With the worldwide introduction and use of the polymerase chain reaction (PCR) methodologies, the detection of different pathogens improved significantly - however, these systems have weak points as well. The parallel screening of more than a few pathogens is not resolved and, in general, the multiplexing capacity of most of the methods used in this area is insufficient. In the case of the detection of pathogens causing similar symptoms (like the vesicular complex, involving Foot-and-Mouth Disease (FMD), Swine Vesicular Disease (SVD) and Vesicular Stomatitis (VS), the immediate differential diagnosis is essential, not only regarding the multiplexing, but also because of the high economic risks and the strict legislations. Subtyping of the avian influenza viruses is a broad and extensive task because it needs to differentiate 16 hemagglutinin and 9 neuraminidase types and their variants. The padlock probes, as used in these studies, seem to be optimal to fulfill the multiplexing requirements and to provide novel, high-throughput tools for the improved diagnosis of the vesicular complex and of avian influenza. The general detection and subtyping of Hepatitis E Virus (HEV) is an important and complicated task today, as the virus shows zoonotic potential by causing endemics in humans and persisting infections in different animal species. Thus, there is a high need for sensitive and specific methods and identification of HEV variants. In the frame of this work, a highly specific and sensitive diagnostic assay was developed, based on two types of real-time PCR methods. In addition, a genotyping system was constructed using a simple and quick ligation-based technique.

Authors/Creators:Gyarmati, Péter
Title:Implementation of molecular detection techniques in the field of veterinary virology
Subtitle:with special reference to the ligation-based methodologies
Year of publishing :2008
Volume:2008:83
Number of Pages:40
Papers/manuscripts:
NumberReferences
ALLI Banér, J., Gyarmati, P., Yacoub, A., Hakhverdyan, M., Stenberg, J., Ericsson, O., Nilsson, M., Landegren, U. & Belák, S. (2007). Microarray-based molecular detection of foot-and-mouth disease, vesicular stomatitis and swine vesicular disease viruses, using padlock probes. J Virol Methods. 143(2):200-6. II Gyarmati, P., Conze, T., Zohari, S., LeBlanc, N., Nilsson, M., Landegren, U., Banér, J. & Belák, S. (2008). Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses using padlock probes. J Clin Microbiol 46(5):1747-51. III Gyarmati, P., Mohammed N., Norder H., Blomberg J., Belák, S. & Widén, F. (2007). Universal detection of hepatitis E virus by two real-time PCR assays: TaqMan and Primer-Probe Energy Transfer. J Virol Methods 146(1-2):226-35. IV Gyarmati, P., Belák, S. & Widén, F. (2008). Genotyping of Hepatitis E virus using ligation-dependent probe amplification. (Manuscript)
Place of Publication:Uppsala
ISBN for printed version:978-91-86195-16-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:avian influenza virus, hepatitis, vesiculovirus, zoonoses, pathogens, nucleid probes, PCR, diagnosis, molecular genetics, virology
Keywords:padlock probes, PCR, microarray, vesicular complex, HEV, avian influenza
URN:NBN:urn:nbn:se:slu:epsilon-2619
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2619
ID Code:1885
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Peter Gyarmati
Deposited On:20 Nov 2008 00:00
Metadata Last Modified:02 Dec 2014 10:14

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