Charova, Spyridoula N. and Dolfors, Fredrik and Holmquist, Louise and Moschou, Panagiotis Nikolaou and Dixelius, Christina and Tzelepis, Georgios
(2020).
The RsRlpA Effector Is a Protease Inhibitor Promoting Rhizoctonia solani Virulence through Suppression of the Hypersensitive Response.
International Journal of Molecular Sciences. 21
, 8070
[Journal article]
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Abstract
Rhizoctonia solani (Rs) is a soil-borne pathogen with a broad host range. This pathogen incites a wide range of disease symptoms. Knowledge regarding its infection process is fragmented, a typical feature for basidiomycetes. In this study, we aimed at identifying potential fungal effectors and their function. From a group of 11 predicted single gene effectors, a rare lipoprotein A (RsRlpA), from a strain attacking sugar beet was analyzed. The RsRlpA gene was highly induced upon early-stage infection of sugar beet seedlings, and heterologous expression in Cercospora beticola demonstrated involvement in virulence. It was also able to suppress the hypersensitive response (HR) induced by the Avr4/Cf4 complex in transgenic Nicotiana benthamiana plants and functioned as an active protease inhibitor able to suppress Reactive Oxygen Species (ROS) burst. This effector contains a double-psi beta-barrel (DPBB) fold domain, and a conserved serine at position 120 in the DPBB fold domain was found to be crucial for HR suppression. Overall, R. solani seems to be capable of inducing an initial biotrophic stage upon infection, suppressing basal immune responses, followed by a switch to necrotrophic growth. However, regulatory mechanisms between the different lifestyles are still unknown.
Authors/Creators: | Charova, Spyridoula N. and Dolfors, Fredrik and Holmquist, Louise and Moschou, Panagiotis Nikolaou and Dixelius, Christina and Tzelepis, Georgios | ||||||
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Title: | The RsRlpA Effector Is a Protease Inhibitor Promoting Rhizoctonia solani Virulence through Suppression of the Hypersensitive Response | ||||||
Year of publishing : | 2020 | ||||||
Volume: | 21 | ||||||
Article number: | 8070 | ||||||
Number of Pages: | 17 | ||||||
ISSN: | 1661-6596 | ||||||
Language: | English | ||||||
Publication Type: | Journal article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science | ||||||
Keywords: | basidiomycete, biotrophy, effector, cathepsin, hypersensitive response, papain inhibitor, protease, soil-borne pathogen, sugar beet | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-108782 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108782 | ||||||
Additional ID: |
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ID Code: | 19070 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) (NL, NJ) > Dept. of Forest Mycology and Plant Pathology (S) > Dept. of Forest Mycology and Plant Pathology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 01 Dec 2020 10:43 | ||||||
Metadata Last Modified: | 15 Jan 2021 19:19 |
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