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The RsRlpA Effector Is a Protease Inhibitor Promoting Rhizoctonia solani Virulence through Suppression of the Hypersensitive Response

Charova, Spyridoula N. and Dolfors, Fredrik and Holmquist, Louise and Moschou, Panagiotis Nikolaou and Dixelius, Christina and Tzelepis, Georgios (2020). The RsRlpA Effector Is a Protease Inhibitor Promoting Rhizoctonia solani Virulence through Suppression of the Hypersensitive Response. International Journal of Molecular Sciences. 21 , 8070
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Abstract

Rhizoctonia solani (Rs) is a soil-borne pathogen with a broad host range. This pathogen incites a wide range of disease symptoms. Knowledge regarding its infection process is fragmented, a typical feature for basidiomycetes. In this study, we aimed at identifying potential fungal effectors and their function. From a group of 11 predicted single gene effectors, a rare lipoprotein A (RsRlpA), from a strain attacking sugar beet was analyzed. The RsRlpA gene was highly induced upon early-stage infection of sugar beet seedlings, and heterologous expression in Cercospora beticola demonstrated involvement in virulence. It was also able to suppress the hypersensitive response (HR) induced by the Avr4/Cf4 complex in transgenic Nicotiana benthamiana plants and functioned as an active protease inhibitor able to suppress Reactive Oxygen Species (ROS) burst. This effector contains a double-psi beta-barrel (DPBB) fold domain, and a conserved serine at position 120 in the DPBB fold domain was found to be crucial for HR suppression. Overall, R. solani seems to be capable of inducing an initial biotrophic stage upon infection, suppressing basal immune responses, followed by a switch to necrotrophic growth. However, regulatory mechanisms between the different lifestyles are still unknown.

Authors/Creators:Charova, Spyridoula N. and Dolfors, Fredrik and Holmquist, Louise and Moschou, Panagiotis Nikolaou and Dixelius, Christina and Tzelepis, Georgios
Title:The RsRlpA Effector Is a Protease Inhibitor Promoting Rhizoctonia solani Virulence through Suppression of the Hypersensitive Response
Series Name/Journal:International Journal of Molecular Sciences
Year of publishing :2020
Volume:21
Article number:8070
Number of Pages:17
ISSN:1661-6596
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:basidiomycete, biotrophy, effector, cathepsin, hypersensitive response, papain inhibitor, protease, soil-borne pathogen, sugar beet
URN:NBN:urn:nbn:se:slu:epsilon-p-108782
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108782
Additional ID:
Type of IDID
DOI10.3390/ijms21218070
Web of Science (WoS)000588986700001
ID Code:19070
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:01 Dec 2020 10:43
Metadata Last Modified:15 Jan 2021 19:19

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