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Genome-wide identification of Argonautes in Solanaceae with emphasis on potato

Liao, Zhen and Persson Hodén, Kristian and Singh, Ravi Kumar and Dixelius, Christina (2020). Genome-wide identification of Argonautes in Solanaceae with emphasis on potato. Scientific Reports. 10 , 20577
[Research article]

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Abstract

Regulatory small RNAs (sRNAs) play important roles in many fundamental processes in plant biology such as development, fertilization and stress responses. The AGO protein family has here a central importance in gene regulation based on their capacity to associate with sRNAs followed by mRNA targeting in a sequence-complementary manner. The present study explored Argonautes (AGOs) in the Solanaceae family, with emphasis on potato, Solanum tuberosum (St). A genome-wide monitoring was performed to provide a deeper insight into gene families, genomic localization, gene structure and expression profile against the potato late blight pathogen Phytophthora infestans. Among 15 species in the Solanaceae family we found a variation from ten AGOs in Nicotiana obtusifolia to 17 in N. tabacum. Comprehensive analyses of AGO phylogeny revealed duplication of AGO1, AGO10 and AGO4 paralogs during early radiation of Solanaceae. Fourteen AGOs were identified in potato. Orthologs of AGO8 and AGO9 were missing in the potato genome. However, AGO15 earlier annotated in tomato was identified. StAGO15 differs from the other paralogs having residues of different physico-chemical properties at functionally important amino acid positions. Upon pathogen challenge StAGO15 was significantly activated and hence may play a prominent role in sRNA-based regulation of potato defense.

Authors/Creators:Liao, Zhen and Persson Hodén, Kristian and Singh, Ravi Kumar and Dixelius, Christina
Title:Genome-wide identification of Argonautes in Solanaceae with emphasis on potato
Series Name/Journal:Scientific Reports
Year of publishing :2020
Volume:10
Article number:20577
Number of Pages:10
ISSN:2045-2322
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
URN:NBN:urn:nbn:se:slu:epsilon-p-108951
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108951
Additional ID:
Type of IDID
DOI10.1038/s41598-020-77593-y
ID Code:19078
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:01 Dec 2020 13:43
Metadata Last Modified:15 Jan 2021 19:26

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