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Genetic structure of a germplasm for hybrid breeding in rye (Secale cereale L.)

Vendelbo, Nikolaj M. and Sarup, Pernille and Orabi, Jihad and Kristensen, Peter S. and Jahoor, Ahmed (2020). Genetic structure of a germplasm for hybrid breeding in rye (Secale cereale L.). PLoS ONE. 15 , e0239541
[Journal article]

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Abstract

Rye (Secale cereale L.) responds strongly to changes in heterozygosity with hybrids portraying strong heterosis effect on all developmental and yielding characteristics. In order to achieve the highest potential heterosis effect parental lines must originate from genetically distinct gene pools. Here we report the first comprehensive SNP-based population study of an elite germplasm using fertilization control system for hybrid breeding in rye that is genetically different to the predominating P-type. In total 376 inbred lines from Nordic Seed Germany GmbH were genotyped for 4419 polymorphic SNPs. The aim of this study was to confirm and quantify the genetic separation of parental populations, unveil their genetic characteristics and investigate underlying population structures. Through a palette of complimenting analysis, we confirmed a strong genetic differentiation (F-ST = 0.332) of parental populations validating the germplasms suitability for hybrid breeding. These were, furthermore, found to diverge considerably in several features with the maternal population portraying a strong population structure characterized by a narrow genetic profile, small effective population size and high genome-wise linkage disequilibrium. We propose that the employed male-sterility system putatively constitutes a population determining parameter by influencing the rate of introducing novel genetic variation to the parental populations. Functional analysis of linkage blocks led to identification of a conserved segment on the distal 4RL chromosomal region annotated to the Rfp3 male-fertility restoration 'Pampa' type gene. Findings of our study emphasized the immediate value of comprehensive population studies on elite breeding germplasms as a pre-requisite for application of genomic-based breeding techniques, introgression of novel material and to support breeder decision-making.

Authors/Creators:Vendelbo, Nikolaj M. and Sarup, Pernille and Orabi, Jihad and Kristensen, Peter S. and Jahoor, Ahmed
Title:Genetic structure of a germplasm for hybrid breeding in rye (Secale cereale L.)
Series Name/Journal:PLoS ONE
Year of publishing :2020
Volume:15
Article number:e0239541
Number of Pages:23
Publisher:PUBLIC LIBRARY SCIENCE
ISSN:1932-6203
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
URN:NBN:urn:nbn:se:slu:epsilon-p-109004
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109004
Additional ID:
Type of IDID
DOI10.1371/journal.pone.0239541
Web of Science (WoS)000581817500046
ID Code:19096
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:03 Dec 2020 11:23
Metadata Last Modified:15 Jan 2021 19:47

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