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Neuromodulation in the chemosensory system of mosquitoes - neuroanatomy and physiology

Siju, K.P. (2009). Neuromodulation in the chemosensory system of mosquitoes - neuroanatomy and physiology. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2009:24
ISBN 978-91-86195-71-7
[Doctoral thesis]

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The impact of mosquito vectored disease on global public health is overwhelming. Mosquitoes depend on chemosensory capability for blood-feeding and as such have a highly developed chemosensory system, which consists of peripheral and central components. The repertoire of mosquito behaviors during their life cycle are modified by external and internal factors. The main goal of this thesis is to describe some factors that modulate odor-associated behavior, focusing on biogenic amines, neuropeptides, and physiological state change. I generated a detailed distribution map of serotonin, one of the major biogenic amines in the central and peripheral chemosensory system of Aedes aegypti. The arborization pattern of serotonin-immunoreactive (SI) neurons in the central chemosensory neuropil included the antennal lobe (AL), subesophageal ganglion, tritocerebrum and higher brain centers. In addition, I found SI fibers in the peripheral chemosensory organs: the antennae, the maxillary palps and the labia. Furthermore, to investigate the affects of the gonotrophic and circadian state of mosquitoes on serotonin, dopamine and octopamine levels in the heads of female Ae. aegypti, I used high-performance liquid chromatography coupled with electrochemical detection. Changes in the titer level of these biogenic amines are correlated to flight activity and physiological status of this mosquito. The neuropeptides in the mosquito AL are a highly diverse class of neurochemicals described by matrix assisted laser desorption ionization time-of-flight mass spectrometry of a single AL. I isolated and identified a total of 26 neuropeptides belonging to 10 gene families. Additionally, the cellular distribution of four major families of neuropeptides revealed distinct localization patterns of each of these families in the AL and to other olfactory-associated neuropil areas in the brain. Finally, I employed single sensillum recording to investigate the modulation of odorant receptor neurons housed in the antennal sensilla trichodea after a blood meal. Three functional classes of short blunt-tipped type II sensilla displayed a higher sensitivity after blood feeding to the host and oviposition-associated cues, indole and three phenolic compounds. This study indicates modulation of olfactory behavior occurs at the peripheral level post blood-meal, which may be associated with the physiological status of the mosquitoes.

Authors/Creators:Siju, K.P.
Title:Neuromodulation in the chemosensory system of mosquitoes - neuroanatomy and physiology
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2009
Number of Pages:68
ALLI Siju, K.P., Hansson, B.S. & Ignell, R. Immunocytochemical localization of serotonin in the central and peripheral chemosensory system of mosquitoes. Arthropod Structure and Development 37, 248-259. II Siju, K.P., Gramsbergen, J.B., Hansson, B.S. & Ignell. Quantification of biogenic amines in female yellow fever mosquitoes, Aedes aegypti, throughout the first gonotrophic cycle. Submitted III Siju, K.P., Schachtner, J., Scheiblich, H., Neupert, S., Predel, R., Hansson, B.S. & Ignell, R. Neuropeptides in the antennal lobe of the yellow fever mosquito, Aedes aegypti. Manuscript IV Siju, K.P., Hill, S.R., Hansson, B.S. & Ignell, R. Influence of blood meal on the responsiveness of olfactory receptor neurons in antennal sensilla trichodea of the yellow fever mosquito, Aedes aegypti. Manuscript
Place of Publication:Alnarp
ISBN for printed version:978-91-86195-71-7
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L50 Animal physiology and biochemistry
Subjects:Not in use, please see Agris categories
Agrovoc terms:pest insects, neurophysiology, olfaction, vectorbourne diseases, pest insects culicidae, biogenic amines, animal morphology
Keywords:olfaction, disease vector, biogenic amines, neuropeptides, neuromodulation, anatomy, electrophysiology
Permanent URL:
ID Code:1956
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: Siju K.P.
Deposited On:06 Mar 2009 00:00
Metadata Last Modified:02 Dec 2014 10:15

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