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Carbon partitioning between starch and oil in Avena sativa (oat) and Arabidopsis thaliana

method development and biochemical studies paving the way for future oil crops

Grimberg, Åsa (2009). Carbon partitioning between starch and oil in Avena sativa (oat) and Arabidopsis thaliana. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2009:26
ISBN 978-91-86195-73-1
[Doctoral thesis]

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In recent years, the demand for plant-derived oils as renewable alternatives to fossil oil has increased due to the rising cost of petroleum and the increased concern about the environment. However, the supply of vegetable oils today relies upon only a few crops with low potential for further increases in oil yields. Redirection of carbon flux from starch to oil in the cereal seed can provide for new high-yielding oil crops as a sustainable alternative to fossil oil to meet the increased need for plant oils. Increased knowledge of oil biochemistry and the regulation of carbon partitioning between different storage compounds in seeds is therefore of crucial importance for the development of such novel oil crops in the future. In a study to determine if the future environment with elevated atmospheric CO2 can be expected to alter the partitioning of carbon into oil in seeds, it was found that elevated CO2 induced a much higher starch accumulation in leaves of A. thaliana whereas seed oil content was unaffected. However, leaf lipid metabolism was altered in an elevated CO2 environment and changes were targeted to the lipids in the photosynthetic membranes of the chloroplasts. Among the cereals, oat is unique in storing high amounts of oil in the endosperm. The deposition of storage compounds during seed development and their mobilization during germination was investigated. There were large differences in partitioning of carbon between oil and carbohydrate reserves between oat cultivars having different oil content, both during seed development and germination. The data suggests that oat is suitable as a model plant for oil synthesis in the cereal endosperm to find the metabolic switches for carbon partitioning between starch and oil. An in vitro liquid culture for oat seeds on detached panicles was established to mimic seed development and cultivar differences in storage accumulation from anthesis to maturity in planta. The in vitro system was used in preliminary stable radioactive isotope labeling experiments that confirmed the cultivar differences in carbon partitioning into oil observed in planta.

Authors/Creators:Grimberg, Åsa
Title:Carbon partitioning between starch and oil in Avena sativa (oat) and Arabidopsis thaliana
Subtitle:method development and biochemical studies paving the way for future oil crops
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2009
Number of Pages:53
ALLI Ekman Å., Bülow L., Stymne S. 2007. Elevated atmospheric CO2 concentration and diurnal cycle induce changes in lipid composition in Arabidopsis thaliana. New Phytologist (174) 591-599. II Banas A., Debski H., Banas W., Heneen W., Dahlvist A., Bafor M., Gummeson P-O., Marttila S., Ekman Å., Carlsson A., Stymne S. 2007. Lipids in grain tissues of oat (Avena sativa): differences in content, time of deposition, and fatty acid composition. Journal of Experimental Botany (58:10) 2463-2470. III Grimberg Å.*, Leonova S. Stymne S., Carlsson A. Mobilization of lipid reserves during germination in two cultivars of oat (Avena sativa) having different amounts of endosperm oil. (Manuscript). IV Ekman Å., Hayden D.M., Dehesh K., Bülow L., Stymne S. 2008. Carbon partitioning between oil and carbohydrates in developing oat (Avena sativa L.) seeds. Journal of Experimental Botany (59:15), 4247-4257. Appendix A. Definitions of developmental stages of oat seeds in planta. Papers I, II, and IV are reproduced with the kind permission from the publishers. *Grimberg Å., maiden name Ekman Å.
Place of Publication:Alnarp
ISBN for printed version:978-91-86195-73-1
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:avena sativa, arabidopsis thaliana, carbon dioxide enrichment, triacylglycerol lipase, germination, endosperm, oats, oil crops, cereal crops
Keywords:Arabidopsis thaliana, Avena sativa, carbon partitioning, cereals, elevated CO2, endosperm, germination, oat, oil crop, starch, triacylglycerol
Permanent URL:
ID Code:1969
Department:(LTJ, LTV) > Plant Breeding and Biotechnology (until 121231)
Deposited By: Åsa Ekman
Deposited On:01 Apr 2009 00:00
Metadata Last Modified:02 Dec 2014 10:15

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