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Marker-Trait Associations for Tolerance to Ash Dieback in Common Ash (Fraxinus excelsior L.)

Chaudhary, Rajiv and Rönneburg, Tilman and Stein Åslund, Matilda and Lundén, Karl and Brandström Durling, Mikael and Ihrmark, Katarina and Menkis, Audrius and Stener, Lars-Göran and Elfstrand, Malin and Cleary, Michelle and Stenlid, Jan (2020). Marker-Trait Associations for Tolerance to Ash Dieback in Common Ash (Fraxinus excelsior L.). Forests. 11 , 1083
[Journal article]

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Abstract

Common ash (Fraxinus excelsior L.) is a tree species of significant ecological and economic importance that has suffered a devastating decline since the 1990s in Europe. Native ash species are being threatened by the alien invasive fungus Hymenoscyphus fraxineus, which causes ash dieback. The main goal of the study was to develop markers for traits related to tolerance to ash dieback and to investigate whether genotypes selected for tolerance were genetically different from susceptible wild populations. We phenotyped 326 ash trees from Sweden for disease severity and genotyped them using 63 amplicon-derived single-nucleotide polymorphism (SNP) markers derived from genes in 40 scaffolds spanning 8 MB in total, which represents approximately 1% of the ash genome. We used a mixed linear model to test for an association between genotypic variation at these loci and disease severity of ash. In total, two SNPs were found to have significant associations. One non-synonymous SNP associated with the disease severity of ash was found in a gene predicted to encode a subtilisin-related peptidase S8/S53 domain. A second marginally significant marker was associated with an LRR gene. Our results demonstrate an inexpensive time-effective method for generating genomic data that could have potential for use in future tree breeding programs and provide information for marker-assisted selection. Our study also showed a low differentiation between genotypes selected for disease tolerance and the wild population of ash representing a range of susceptibilities to ash dieback, indicating opportunities for further selection without significantly losing genetic diversity in the ash population.

Authors/Creators:Chaudhary, Rajiv and Rönneburg, Tilman and Stein Åslund, Matilda and Lundén, Karl and Brandström Durling, Mikael and Ihrmark, Katarina and Menkis, Audrius and Stener, Lars-Göran and Elfstrand, Malin and Cleary, Michelle and Stenlid, Jan
Title:Marker-Trait Associations for Tolerance to Ash Dieback in Common Ash (Fraxinus excelsior L.)
Series Name/Journal:Forests
Year of publishing :2020
Volume:11
Article number:1083
Number of Pages:13
Publisher:MDPI
ISSN:1999-4907
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:common ash, Hymenoscyphus fraxineus, markers, selection for tolerance, association genetics, subtilisin-related peptidase, Marker-assisted selection, Hiplex
URN:NBN:urn:nbn:se:slu:epsilon-p-109313
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109313
Additional ID:
Type of IDID
DOI10.3390/f11101083
Web of Science (WoS)000586964600001
ID Code:19762
Faculty:S - Faculty of Forest Sciences
NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology

(S) > Southern Swedish Forest Research Centre
Deposited By: SLUpub Connector
Deposited On:22 Dec 2020 20:04
Metadata Last Modified:15 Jan 2021 19:19

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