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Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro

Hellman, Stina and Tyden, Eva and Hjertner, Bernt and Nilsfors, Frida and Hu, Kefei and Morein, Bror and Fossum, Caroline (2020). Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro. Parasite Immunology. 43 , e12794
[Journal article]

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Abstract

Aims To generate different larval stages ofStrongylus vulgarisand to study cytokine responses in cultures of eqPBMC exposed to defined larval stages ofS. vulgarisand cyathostomins with the aim to understand the early immune reaction to these parasites. Methods and results EqPBMC were exposed toS. vulgarislarvae (L3, exsheated L3 and L4) and cyathostomin L3 and analysed for cytokine gene expression. Procedures for decontamination, culturing and attenuation of larvae were established. Transcription of IL-4, IL-5 and IL-13 was induced by bothS. vulgarisand cyathostomin L3. Moulting ofS. vulgarisfrom L3 to L4 stage was accompanied by a shift to high expression of IL-5 and IL-9 (exsheated L3 and L4) and IFN-gamma (L4 only). In parallel, the adjuvant G3 modified the cytokine profile induced by both parasites by reducing the expression of IL-4, IL-5 and IL-10 while concomitantly enhancing the expression of IFN-gamma. Conclusion The L4 stage ofS. vulgarisgenerated a cytokine profile different from that induced by the earlier L3 stage ofS. vulgarisand cyathostomins. This diversity depending on the life cycle stage will have implications for the choice of antigen and adjuvant in future vaccine design.

Authors/Creators:Hellman, Stina and Tyden, Eva and Hjertner, Bernt and Nilsfors, Frida and Hu, Kefei and Morein, Bror and Fossum, Caroline
Title:Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro
Year of publishing :2020
Volume:43
Article number:e12794
Number of Pages:10
Publisher:WILEY
ISSN:0141-9838
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
Keywords:adjuvant, cytokine, gene expression, horse, parasite, peripheral blood mononuclear cells
URN:NBN:urn:nbn:se:slu:epsilon-p-108164
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108164
Additional ID:
Type of IDID
DOI10.1111/pim.12794
Web of Science (WoS)000574762500001
ID Code:19825
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:30 Dec 2020 05:43
Metadata Last Modified:15 Jan 2021 19:22

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