Hellman, Stina and Tyden, Eva and Hjertner, Bernt and Nilsfors, Frida and Hu, Kefei and Morein, Bror and Fossum, Caroline
(2020).
Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro.
Parasite Immunology. 43
, e12794
[Research article]
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Abstract
Aims To generate different larval stages ofStrongylus vulgarisand to study cytokine responses in cultures of eqPBMC exposed to defined larval stages ofS. vulgarisand cyathostomins with the aim to understand the early immune reaction to these parasites. Methods and results EqPBMC were exposed toS. vulgarislarvae (L3, exsheated L3 and L4) and cyathostomin L3 and analysed for cytokine gene expression. Procedures for decontamination, culturing and attenuation of larvae were established. Transcription of IL-4, IL-5 and IL-13 was induced by bothS. vulgarisand cyathostomin L3. Moulting ofS. vulgarisfrom L3 to L4 stage was accompanied by a shift to high expression of IL-5 and IL-9 (exsheated L3 and L4) and IFN-gamma (L4 only). In parallel, the adjuvant G3 modified the cytokine profile induced by both parasites by reducing the expression of IL-4, IL-5 and IL-10 while concomitantly enhancing the expression of IFN-gamma. Conclusion The L4 stage ofS. vulgarisgenerated a cytokine profile different from that induced by the earlier L3 stage ofS. vulgarisand cyathostomins. This diversity depending on the life cycle stage will have implications for the choice of antigen and adjuvant in future vaccine design.
| Authors/Creators: | Hellman, Stina and Tyden, Eva and Hjertner, Bernt and Nilsfors, Frida and Hu, Kefei and Morein, Bror and Fossum, Caroline | ||||||
|---|---|---|---|---|---|---|---|
| Title: | Cytokine responses to various larval stages of equine strongyles and modulatory effects of the adjuvant G3 in vitro | ||||||
| Series Name/Journal: | Parasite Immunology | ||||||
| Year of publishing : | 2020 | ||||||
| Volume: | 43 | ||||||
| Article number: | e12794 | ||||||
| Number of Pages: | 10 | ||||||
| Publisher: | WILEY | ||||||
| ISSN: | 0141-9838 | ||||||
| Language: | English | ||||||
| Publication Type: | Research article | ||||||
| Article category: | Scientific peer reviewed | ||||||
| Version: | Published version | ||||||
| Copyright: | Creative Commons: Attribution-Noncommercial 4.0 | ||||||
| Full Text Status: | Public | ||||||
| Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology | ||||||
| Keywords: | adjuvant, cytokine, gene expression, horse, parasite, peripheral blood mononuclear cells | ||||||
| URN:NBN: | urn:nbn:se:slu:epsilon-p-108164 | ||||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108164 | ||||||
| Additional ID: |
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| ID Code: | 19825 | ||||||
| Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
| Department: | (VH) > Department of Biomedical Science and Veterinary Public Health | ||||||
| Deposited By: | SLUpub Connector | ||||||
| Deposited On: | 30 Dec 2020 05:43 | ||||||
| Metadata Last Modified: | 15 Jan 2021 19:22 |
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