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Active oxygen involvement in developmental processes in Populus

with emphasis on HipI-superoxide dismutase

Srivastava, Vaibhav (2009). Active oxygen involvement in developmental processes in Populus. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2009:21
ISBN 978-91-86195-68-7
[Doctoral thesis]

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Abstract

In plants, oxidative stress is result of disruption of the cellular redox metabolism and is caused by a variety of stress conditions (abiotic and/or biotic). This leads to the induction of several mechanisms that protect against disruption of the redox balance, as well as mechanisms to assist in recovery from toxicity/damage caused by increased cellular levels of reactive oxygen species (ROS). The superoxide dismutase enzymes (SODs) are key component of the reactive oxygen species gene network and represent the first line of defense against ROS, by converting superoxide radicals (O2−) to hydrogen peroxide (H2O2) and water (H2O). Therefore, SODs play an important role in protection against oxidative stress in all aerobic organisms. In this thesis, I describe the characterization of an SOD isoform from Populus, hipI-SOD, which has a high iso-electric point. The global response to oxidative stress is also discussed. Different forms of hipI-SOD transcripts were found in vascular tissue, one of which was produced by alternative splicing. HipI-SOD proteins were found to be mainly localized extracellularly, in the primary and secondary cell walls of vascular tissues. These results together with analysis of transgenic Populus trees with suppressed expression of hipI-SOD strongly indicate roles for hipI-SOD in regulating ROS levels in vascular tissue. ROS are important regulators of plant stress responses. Nevertheless, oxidative stress often affects plants growth and development. In order to understand the basis of oxidative stress tolerance, the diversity of stress responses needs to be investigated. To achieve this we first developed an O2PLS-based multivariate methodology for the integration of multiple datasets originating from three different platforms (transcriptomics, proteomics and metabolomics). Subsequently this data integration method was utilized for a comprehensive study of the overall responses to oxidative stress in Populus. The findings may facilitate the development of stress-tolerant plants with improved survival rates and yields under stressed conditions.

Authors/Creators:Srivastava, Vaibhav
Title:Active oxygen involvement in developmental processes in Populus
Subtitle:with emphasis on HipI-superoxide dismutase
Year of publishing :2009
Volume:2009:21
Number of Pages:61
Papers/manuscripts:
NumberReferences
ALL[I]Srivastava V, Srivastava MK, Chibani K, Nilsson R, Nicolas R, Melzer M. and Wingsle G. (2009) Alternative splicing studies of reactive oxygen species gene network in Populus reveal two isoforms of high-isoelectric-point Superoxide Dismutase. Plant Physiology 149, 1848-1859 [II]Srivastava V, Schinkel H, Witzell J, Hertzberg M, Torp M, Srivastava MK, Karpinska B, Melzer M. and Wingsle G. (2007) Downregulation of high-isoelectric-point extracellular superoxide dismutase mediates alterations in the metabolism of reactive oxygen species and developmental disturbances in hybrid aspen. The Plant Journal 49, 135-148 [III]Bylesjö M, Nilsson R, Srivastava V, Grönlund A, Johansson AI, Jansson S, Karlsson J, Moritz M, Wingsle G. and Trygg J. (2009) Integrated analysis of transcript, protein and metabolite data to study lignin biosynthesis in Hybrid Aspen. Journal of Proteome Research 8, 199-210 [IV]Srivastava V, Hvidsten TR, Ryden P, Nilsson R, Freyhult E, Bygdell J, Quarnström J, Moritz T, Karlsson J, Trygg J. and Wingsle G. A Systems Biology approach to studying global responses to oxidative stress in Populus. Manuscript
Place of Publication:Umeå
ISBN for printed version:978-91-86195-68-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:populus, superoxide dismutase, vascular tissues, cell walls, oxidation, stress
Keywords:alternative splicing, Populus, superoxide dismutase, hipI-SOD, vascular tissue, cell wall, reactive oxygen species, oxidative stress, O2PLS
URN:NBN:urn:nbn:se:slu:epsilon-2873
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2873
ID Code:2008
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: Vaibhav Srivastava
Deposited On:14 May 2009 00:00
Metadata Last Modified:02 Dec 2014 10:15

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