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Towards the domestication of Lepidium campestre as an undersown oilseed crop

Eriksson, Dennis (2009). Towards the domestication of Lepidium campestre as an undersown oilseed crop. Diss. (sammanfattning/summary) Epsilon : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2009:65
ISBN 978-91-576-7412-8
[Doctoral thesis]

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Abstract

Lepidium campestre (field cress) has been selected for domestication. It is proposed to be cultivated as a catch crop in a spring cereal and produce a seed oil of industrial quality the following year(s). This crop system will help to alleviate the problem of nutrient leaching from agricultural soils. The oil produce will provide an alternative to fossil oil in certain industrial applications. This thesis addresses the issues of seed dispersal, seed oil quality and also includes a field trial evaluation of L. campestre. A shoot regeneration protocol has been developed for L. campestre in order to enable a transformation procedure. The most important factors include a pretreatment on 2,4-D and a temporary starvation treatment. The combination of plant growth hormones in the regeneration medium was of little importance. For transformation, a number of factors for co-cultivation with Agrobacterium tumefaciens have been evaluated. So far, however, there has been little success. Microscopical studies on L. campestre fruits have identified a basic architecture as well as a dehiscence zone (DZ) that are important for seed dispersal. A gene controlling the development of the DZ, the INDEHISCENT, has been cloned from L. campestre. The function of the gene has been confirmed through functional complementation of the corresponding mutant, as well as RNAi down regulation, in A. thaliana. To modify the seed oil quality, the genes encoding two important enzymes, the FATTY ACID DESATURASE 2 and the FATTY ACID ELONGASE 1, have been cloned from L. campestre. The functions of these genes have been confirmed through functional complementation of the corresponding A. thaliana mutants. A three-year field trial with L. campestre undersown in spring barley demonstrated the high seed yield potential of this species. A positive effect on the barley yield was recorded when L. campestre was undersown with short row distance.

Authors/Creators:Eriksson, Dennis
Title:Towards the domestication of Lepidium campestre as an undersown oilseed crop
Year of publishing :2009
Volume:2009:65
Number of Pages:42
Papers/manuscripts:
NumberReferences
ALLEriksson D, Merker A. 2009. An efficient adventitious shoot regeneration protocol for Lepidium campestre (L.) R. BR.. Propagation of Ornamental Plants (9:2), 78-83. Eriksson D, Merker A. 2009. Identification of a gene involved in pod shatter in the novel oil crop Lepidium campestre. (Manuscript). Eriksson D, Merker A. 2009. Lepidium campestre FAD2 and FAE1 cDNAs complement the corresponding fad2 and fae1 mutations in transgenic Arabidopsis plants. (Manuscript). Merker A, Eriksson D, Bertholdsson N-O. 2009. Barley yield increases with undersown Lepidium campestre. Acta Agriculturae Scandinavica, Section B – Soil and Plant Science, published online 13 July.
Place of Publication:Epsilon
ISBN for printed version:978-91-576-7412-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:lepidium, domestication, oil crops, catch crops, arabidopsis thaliana, shoots, fatty acids, undersowing
Keywords:Lepidium campestre, Arabidopsis thaliana, domestication, oil crop, perennial, pod shatter, fatty acid, shoot regeneration, transformation
URN:NBN:urn:nbn:se:slu:epsilon-2963
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2963
ID Code:2107
Department:(LTJ, LTV) > Plant Breeding and Biotechnology (until 121231)
Deposited By: Dennis Eriksson
Deposited On:15 Sep 2009 00:00
Metadata Last Modified:02 Dec 2014 10:16

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