Hoang, Thomas K and Freeborn, Jasmin and Wang, Ting and Mai, Tu and He, Baokun and Park, Sinyoung and Tran, Dat Q. and Roos, Stefan and Rhoads, J. Marc and Liu, Yuying
(2019).
Human breast milk promotes the immunomodulatory function of probiotic Lactobacillus reuteri DSM 17938 in the neonatal rat intestine.
Journal of probiotics & health. 7
, 1000210
[Journal article]
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Abstract
Background and objective: Breast milk has many growth-promoting and immune-active components, including transforming growth factor-β, lactoferrin, lysozyme, immunoglobulin A, and prebiotics such as the human milk oligosaccharides. Treatment with Lactobacillus reuteri DSM 17938 (LR), a probiotic with immunomodulatory functions, significantly increases regulatory T cells (Tregs) in the intestinal mucosa of newborn suckling rats. In humans, treatment with LR of infants with colic reduces crying optimally if the infants are breast-fed. Therefore, we examined the effects of human breast milk (HBM) on LR-associated immune modulation.
Methods: Newborn rats were divided into 8 feeding groups, including dam-fed ± LR (106 CFU/kg bw/day, daily), formula-fed ± LR, formula with 20% (v/v) HBM-fed ± LR, and HBM-fed ± LR. Pups were fed by gavage from d1 to d3 of age. Subsequently, we measured intestinal immune cell profiles, including Tregs and tolerogenic dendritic cells (tDCs) by flow cytometry. We also measured inflammatory cytokine and chemokine levels of interleukin (IL)-1β and cytokine-induced neutrophil chemoattratant (CINC)-1 in intestinal tissue lysates by ELISA.
Results and Conclusion: (1) Formula feeding increased intestinal CD3+ T cells, CD4+ helper T (TH) cells and CD11c+ DCs, pro-inflammatory effects which were reversed by HBM. (2) When comparing HBM-fed with formulafed newborns, HBM supplementation produced a lower percentage of CD4+ TH cells and a higher percentage of CD8+ (cytotoxic) T cells, while reducing protein levels of IL-1β and CINC-1 in the intestine. (3) Probiotic LR feeding maximally stimulated the percentage of intestinal Tregs and tDCs when the pups were fed HBM. In conclusion, HBM reduced formula-induced intestinal gut immune activation, and the addition of LR further promoted immune tolerance.
Authors/Creators: | Hoang, Thomas K and Freeborn, Jasmin and Wang, Ting and Mai, Tu and He, Baokun and Park, Sinyoung and Tran, Dat Q. and Roos, Stefan and Rhoads, J. Marc and Liu, Yuying | ||||
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Title: | Human breast milk promotes the immunomodulatory function of probiotic Lactobacillus reuteri DSM 17938 in the neonatal rat intestine | ||||
Year of publishing : | 2019 | ||||
Volume: | 7 | ||||
Article number: | 1000210 | ||||
Number of Pages: | 8 | ||||
ISSN: | 2329-8901 | ||||
Language: | English | ||||
Publication Type: | Journal article | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Immunology in the medical area | ||||
Keywords: | Probiotic, Lactobacillus reuteri, Neonatal rats, T cell, Dendritic cell, Formula, Human breast milk, Intestine | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-109406 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109406 | ||||
Additional ID: |
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ID Code: | 21115 | ||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||
Department: | (NL, NJ) > Department of Molecular Sciences | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 08 Jan 2021 07:04 | ||||
Metadata Last Modified: | 08 Jan 2021 07:11 |
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