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Human breast milk promotes the immunomodulatory function of probiotic Lactobacillus reuteri DSM 17938 in the neonatal rat intestine

Hoang, Thomas K and Freeborn, Jasmin and Wang, Ting and Mai, Tu and He, Baokun and Park, Sinyoung and Tran, Dat Q. and Roos, Stefan and Rhoads, J. Marc and Liu, Yuying (2019). Human breast milk promotes the immunomodulatory function of probiotic Lactobacillus reuteri DSM 17938 in the neonatal rat intestine. Journal of probiotics & health. 7 , 1000210
[Journal article]

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Abstract

Background and objective: Breast milk has many growth-promoting and immune-active components, including transforming growth factor-β, lactoferrin, lysozyme, immunoglobulin A, and prebiotics such as the human milk oligosaccharides. Treatment with Lactobacillus reuteri DSM 17938 (LR), a probiotic with immunomodulatory functions, significantly increases regulatory T cells (Tregs) in the intestinal mucosa of newborn suckling rats. In humans, treatment with LR of infants with colic reduces crying optimally if the infants are breast-fed. Therefore, we examined the effects of human breast milk (HBM) on LR-associated immune modulation.
Methods: Newborn rats were divided into 8 feeding groups, including dam-fed ± LR (106 CFU/kg bw/day, daily), formula-fed ± LR, formula with 20% (v/v) HBM-fed ± LR, and HBM-fed ± LR. Pups were fed by gavage from d1 to d3 of age. Subsequently, we measured intestinal immune cell profiles, including Tregs and tolerogenic dendritic cells (tDCs) by flow cytometry. We also measured inflammatory cytokine and chemokine levels of interleukin (IL)-1β and cytokine-induced neutrophil chemoattratant (CINC)-1 in intestinal tissue lysates by ELISA.
Results and Conclusion: (1) Formula feeding increased intestinal CD3+ T cells, CD4+ helper T (TH) cells and CD11c+ DCs, pro-inflammatory effects which were reversed by HBM. (2) When comparing HBM-fed with formulafed newborns, HBM supplementation produced a lower percentage of CD4+ TH cells and a higher percentage of CD8+ (cytotoxic) T cells, while reducing protein levels of IL-1β and CINC-1 in the intestine. (3) Probiotic LR feeding maximally stimulated the percentage of intestinal Tregs and tDCs when the pups were fed HBM. In conclusion, HBM reduced formula-induced intestinal gut immune activation, and the addition of LR further promoted immune tolerance.

Authors/Creators:Hoang, Thomas K and Freeborn, Jasmin and Wang, Ting and Mai, Tu and He, Baokun and Park, Sinyoung and Tran, Dat Q. and Roos, Stefan and Rhoads, J. Marc and Liu, Yuying
Title:Human breast milk promotes the immunomodulatory function of probiotic Lactobacillus reuteri DSM 17938 in the neonatal rat intestine
Year of publishing :2019
Volume:7
Article number:1000210
Number of Pages:8
ISSN:2329-8901
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Immunology in the medical area
Keywords:Probiotic, Lactobacillus reuteri, Neonatal rats, T cell, Dendritic cell, Formula, Human breast milk, Intestine
URN:NBN:urn:nbn:se:slu:epsilon-p-109406
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109406
Additional ID:
Type of IDID
DOI10.35248/2329-8901.19.7.210
ID Code:21115
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:08 Jan 2021 07:04
Metadata Last Modified:08 Jan 2021 07:11

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