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Lead contamination alters enzyme activities and microbial composition in the rhizosphere soil of the hyperaccumulator Pogonatherum crinitum

Hou, Xiao-long and Han, Hang and Tigabu, Mulualem and Li, Qi-yan and Zhu, Chen-lu and Huang, Si-qi and Cai, Li-ping and Liu, Ai-qin (2021). Lead contamination alters enzyme activities and microbial composition in the rhizosphere soil of the hyperaccumulator Pogonatherum crinitum. Ecotoxicology and Environmental Safety. 207 , 111308
[Research article]

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Abstract

Pogonatherum crinitum is a promising lead (Pb) hyperaccumulator; however, the effects of Pb contamination on P. crinitum rhizosphere soil enzymatic activities and microbial composition remain largely unexplored. Thus, an indoor experiment was conducted by cultivating P. crinitum seedlings and exposing them to four Pb concentrations (0, 1,000, 2000 and 3000 mg/kg Pb). Protease, urease, acid phosphatase and invertase activities were determined using standard methods while soil bacterial composition was determined by 16 S rDNA sequencing. The results showed that rhizosphere soil acid phosphatase activity significantly increased with increasing Pb concentration, while urease activity was significantly greater in rhizosphere soil contaminated with 1000 and 2000 mg/kg than in the control. There was a clear shift in bacterial composition during phytoremediation by P. crinitum. Compared to the control, Bacteroidetes was more abundant in all Pb-contaminated soils, Actinobacteria was more abundant in 1000 mg/kg Pb-treated soil, and Firmicutes was more abundant in 3000 mg/kg Pb-treated soil. Positive correlations were observed between dominant bacterial phyla and soil enzyme activities. Metabolic pathways, such as ABC transporter, quinine reductase, and ATP-binding protein were significantly increased in rhizosphere soil bacteria with Pb contamination. In conclusion, Pb contamination differentially influenced the activities of rhizosphere soil enzymes, specifically increasing acid phosphatase and urease activities, and alters the dominance of soil bacteria through up-regulation of genes related to some metabolic pathways. The strong correlations between dominant bacterial phyla and enzymatic activities suggest synergetic effects on the growth of P. crinitum during Pb contamination.

Authors/Creators:Hou, Xiao-long and Han, Hang and Tigabu, Mulualem and Li, Qi-yan and Zhu, Chen-lu and Huang, Si-qi and Cai, Li-ping and Liu, Ai-qin
Title:Lead contamination alters enzyme activities and microbial composition in the rhizosphere soil of the hyperaccumulator Pogonatherum crinitum
Series Name/Journal:Ecotoxicology and Environmental Safety
Year of publishing :2021
Volume:207
Article number:111308
Number of Pages:8
Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN:0147-6513
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 105 Earth and Related Environmental Sciences > Environmental Sciences (social aspects to be 507)
Keywords:16S rDNA Bacteroidetes, Proteobacteria, protease, urease, Acid phosphatase
URN:NBN:urn:nbn:se:slu:epsilon-p-109726
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109726
Additional ID:
Type of IDID
DOI10.1016/j.ecoenv.2020.111308
Web of Science (WoS)000592461000002
ID Code:21227
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:14 Jan 2021 14:24
Metadata Last Modified:14 Jan 2021 14:31

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