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ACAULIS5 Is Required for Cytokinin Accumulation and Function During Secondary Growth of Populus Trees

Milhinhos, Ana and Bollhoner, Benjamin and Blazquez, Miguel A. and Novak, Ondrej and Miguel, Celia M. and Tuominen, Hannele (2020). ACAULIS5 Is Required for Cytokinin Accumulation and Function During Secondary Growth of Populus Trees. Frontiers in Plant Science. 11 , 601858
[Research article]

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Abstract

In the primary root and young hypocotyl of Arabidopsis, ACAULIS5 promotes translation of SUPPRESSOR OF ACAULIS51 (SAC51) and thereby inhibits cytokinin biosynthesis and vascular cell division. In this study, the relationships between ACAULIS5, SAC51 and cytokinin biosynthesis were investigated during secondary growth of Populus stems. Overexpression of ACAULIS5 from the constitutive 35S promoter in hybrid aspen (Populus tremula x Populus tremuloides) trees suppressed the expression level of ACAULIS5, which resulted in low levels of the physiologically active cytokinin bases as well as their direct riboside precursors in the transgenic lines. Low ACAULIS5 expression and low cytokinin levels of the transgenic trees coincided with low cambial activity of the stem. ACAULIS5 therefore, contrary to its function in young seedlings in Arabidopsis, stimulates cytokinin accumulation and cambial activity during secondary growth of the stem. This function is not derived from maturing secondary xylem tissues as transgenic suppression of ACAULIS5 levels in these tissues did not influence secondary growth. Interestingly, evidence was obtained for increased activity of the anticlinal division of the cambial initials under conditions of low ACAULIS5 expression and low cytokinin accumulation. We propose that ACAULIS5 integrates auxin and cytokinin signaling to promote extensive secondary growth of tree stems.

Authors/Creators:Milhinhos, Ana and Bollhoner, Benjamin and Blazquez, Miguel A. and Novak, Ondrej and Miguel, Celia M. and Tuominen, Hannele
Title:ACAULIS5 Is Required for Cytokinin Accumulation and Function During Secondary Growth of Populus Trees
Series Name/Journal:Frontiers in Plant Science
Year of publishing :2020
Volume:11
Article number:601858
Number of Pages:11
Publisher:FRONTIERS MEDIA SA
ISSN:1664-462X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:ACAULIS5, cytokinin, POPACAULIS5, polyamine, Populus tremula ×Populus tremuloides, thermospermine, wood development, xylem
URN:NBN:urn:nbn:se:slu:epsilon-p-109747
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109747
Additional ID:
Type of IDID
DOI10.3389/fpls.2020.601858
Web of Science (WoS)000593932900001
ID Code:21237
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:15 Jan 2021 09:04
Metadata Last Modified:15 Jan 2021 09:11

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