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The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato

Dominguez-Figueroa, Jose and Carrillo, Laura and Renau-Morata, Begona and Yang, Lu and Molina, Rosa- and Marino, Daniel and Canales, Javier and Weih, Martin and Vicente-Carbajosa, Jesus and Nebauer, Sergio G. and Medina, Joaquin (2020). The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato. Frontiers in Plant Science. 11 , 601558
[Research article]

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Abstract

Nitrate is an essential macronutrient and a signal molecule that regulates the expression of multiple genes involved in plant growth and development. Here, we describe the participation of Arabidopsis DNA binding with one finger (DOF) transcription factor CDF3 in nitrate responses and shows that CDF3 gene is induced under nitrate starvation. Moreover, knockout cdf3 mutant plants exhibit nitrate-dependent lateral and primary root modifications, whereas CDF3 overexpression plants show increased biomass and enhanced root development under both nitrogen poor and rich conditions. Expression analyses of 35S::CDF3 lines reveled that CDF3 regulates the expression of an important set of nitrate responsive genes including, glutamine synthetase-1, glutamate synthase-2, nitrate reductase-1, and nitrate transporters NRT2.1, NRT2.4, and NRT2.5 as well as carbon assimilation genes like PK1 and PEPC1 in response to N availability. Consistently, metabolite profiling disclosed that the total amount of key N metabolites like glutamate, glutamine, and asparagine were higher in CDF3-overexpressing plants, but lower in cdf3-1 in N limiting conditions. Moreover, overexpression of CDF3 in tomato increased N accumulation and yield efficiency under both optimum and limiting N supply. These results highlight CDF3 as an important regulatory factor for the nitrate response, and its potential for improving N use efficiency in crops.

Authors/Creators:Dominguez-Figueroa, Jose and Carrillo, Laura and Renau-Morata, Begona and Yang, Lu and Molina, Rosa- and Marino, Daniel and Canales, Javier and Weih, Martin and Vicente-Carbajosa, Jesus and Nebauer, Sergio G. and Medina, Joaquin
Title:The Arabidopsis Transcription Factor CDF3 Is Involved in Nitrogen Responses and Improves Nitrogen Use Efficiency in Tomato
Series Name/Journal:Frontiers in Plant Science
Year of publishing :2020
Volume:11
Article number:601558
Number of Pages:13
Publisher:FRONTIERS MEDIA SA
ISSN:1664-462X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:CDF, nitrate, tomato, photosynthesis, crop yield, C, N metabolism, transcriptome
URN:NBN:urn:nbn:se:slu:epsilon-p-109981
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109981
Additional ID:
Type of IDID
DOI10.3389/fpls.2020.601558
Web of Science (WoS)000596343000001
ID Code:21277
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Crop Production Ecology
Deposited By: SLUpub Connector
Deposited On:19 Jan 2021 13:43
Metadata Last Modified:19 Jan 2021 13:51

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